Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12910 and PS417_12915 are separated by 70 nucleotides PS417_12915 and PS417_12920 overlap by 13 nucleotides PS417_12920 and PS417_12925 overlap by 4 nucleotides
PS417_12910: PS417_12910 - alanine racemase, at 2,824,606 to 2,825,703
_12910
PS417_12915: PS417_12915 - DNA-binding protein, at 2,825,774 to 2,826,400
_12915
PS417_12920: PS417_12920 - endoribonuclease, at 2,826,388 to 2,826,768
_12920
PS417_12925: PS417_12925 - serine dehydratase, at 2,826,765 to 2,827,730
_12925
Position (kb)
2825
2826
2827 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2824.987 kb on + strand, within PS417_12910 at 2824.988 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand at 2825.762 kb on - strand at 2825.783 kb on - strand at 2825.783 kb on - strand at 2826.083 kb on + strand, within PS417_12915 at 2826.084 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.384 kb on - strand at 2826.389 kb on + strand at 2826.389 kb on + strand at 2826.398 kb on + strand at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.598 kb on + strand, within PS417_12920 at 2826.689 kb on + strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.793 kb on + strand at 2826.794 kb on - strand at 2827.010 kb on - strand, within PS417_12925 at 2827.010 kb on - strand at 2827.129 kb on + strand, within PS417_12925 at 2827.130 kb on - strand, within PS417_12925 at 2827.372 kb on - strand at 2827.374 kb on + strand, within PS417_12925 at 2827.374 kb on + strand, within PS417_12925
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 2,824,987 + PS417_12910 0.35 +2.6 2,824,988 - PS417_12910 0.35 -1.2 2,825,009 - PS417_12910 0.37 -1.5 2,825,009 - PS417_12910 0.37 -0.0 2,825,009 - PS417_12910 0.37 +1.2 2,825,009 - +1.0 2,825,762 - +1.3 2,825,783 - +1.3 2,825,783 - +0.4 2,826,083 + PS417_12915 0.49 -0.8 2,826,084 - PS417_12915 0.49 -0.0 2,826,289 - PS417_12915 0.82 +2.3 2,826,289 - PS417_12915 0.82 -0.8 2,826,384 - -0.5 2,826,389 + +0.2 2,826,389 + +0.6 2,826,398 + -0.0 2,826,539 + PS417_12920 0.40 +0.9 2,826,539 + PS417_12920 0.40 +0.7 2,826,539 + PS417_12920 0.40 -0.5 2,826,540 - PS417_12920 0.40 +0.8 2,826,540 - PS417_12920 0.40 -0.8 2,826,540 - PS417_12920 0.40 -1.0 2,826,598 + PS417_12920 0.55 -0.6 2,826,689 + PS417_12920 0.79 +0.7 2,826,690 - PS417_12920 0.79 -0.3 2,826,690 - PS417_12920 0.79 -1.0 2,826,690 - PS417_12920 0.79 -0.8 2,826,690 - PS417_12920 0.79 +3.2 2,826,793 + -1.0 2,826,794 - -1.1 2,827,010 - PS417_12925 0.25 -0.2 2,827,010 - +1.7 2,827,129 + PS417_12925 0.38 -1.8 2,827,130 - PS417_12925 0.38 +1.3 2,827,372 - +1.3 2,827,374 + PS417_12925 0.63 -0.6 2,827,374 + PS417_12925 0.63 -1.1
Or see this region's nucleotide sequence