Strain Fitness in Pseudomonas simiae WCS417 around PS417_10515

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_10505 and PS417_10510 are separated by 149 nucleotidesPS417_10510 and PS417_10515 are separated by 187 nucleotidesPS417_10515 and PS417_10520 are separated by 11 nucleotidesPS417_10520 and PS417_10525 are separated by 34 nucleotides PS417_10505: PS417_10505 - hypothetical protein, at 2,242,241 to 2,243,089 _10505 PS417_10510: PS417_10510 - esterase, at 2,243,239 to 2,243,655 _10510 PS417_10515: PS417_10515 - acetyl-CoA acetyltransferase, at 2,243,843 to 2,245,024 _10515 PS417_10520: PS417_10520 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,036 to 2,245,695 _10520 PS417_10525: PS417_10525 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,730 to 2,246,428 _10525 Position (kb) 2243 2244 2245 2246Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2243.121 kb on + strandat 2243.204 kb on + strandat 2243.204 kb on + strandat 2243.204 kb on + strandat 2243.204 kb on + strandat 2243.205 kb on - strandat 2243.223 kb on + strandat 2243.256 kb on - strandat 2243.727 kb on + strandat 2243.727 kb on + strandat 2243.728 kb on - strandat 2243.827 kb on + strandat 2243.827 kb on + strandat 2243.828 kb on - strandat 2243.828 kb on - strandat 2244.174 kb on - strand, within PS417_10515at 2244.174 kb on - strand, within PS417_10515at 2244.657 kb on + strand, within PS417_10515at 2244.657 kb on + strand, within PS417_10515at 2244.657 kb on + strand, within PS417_10515at 2244.657 kb on + strand, within PS417_10515at 2244.858 kb on - strand, within PS417_10515at 2245.184 kb on - strand, within PS417_10520at 2245.370 kb on - strand, within PS417_10520at 2245.370 kb on - strand, within PS417_10520at 2245.370 kb on - strand, within PS417_10520at 2245.394 kb on - strand, within PS417_10520at 2245.708 kb on + strandat 2245.731 kb on + strandat 2245.732 kb on - strandat 2245.794 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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2,243,121 + +0.7
2,243,204 + +2.6
2,243,204 + +2.0
2,243,204 + -0.0
2,243,204 + -0.0
2,243,205 - +0.7
2,243,223 + -1.0
2,243,256 - +0.3
2,243,727 + +2.7
2,243,727 + -1.4
2,243,728 - +0.1
2,243,827 + +2.3
2,243,827 + -0.0
2,243,828 - +1.5
2,243,828 - +0.5
2,244,174 - PS417_10515 0.28 -1.4
2,244,174 - PS417_10515 0.28 -0.7
2,244,657 + PS417_10515 0.69 -1.0
2,244,657 + PS417_10515 0.69 -0.5
2,244,657 + PS417_10515 0.69 +3.3
2,244,657 + PS417_10515 0.69 -1.7
2,244,858 - PS417_10515 0.86 +0.1
2,245,184 - PS417_10520 0.22 -1.9
2,245,370 - PS417_10520 0.51 -2.8
2,245,370 - PS417_10520 0.51 +1.3
2,245,370 - PS417_10520 0.51 -3.0
2,245,394 - PS417_10520 0.54 -0.6
2,245,708 + +2.3
2,245,731 + -1.4
2,245,732 - -0.2
2,245,794 + -0.0

Or see this region's nucleotide sequence