Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07635 and PS417_07640 are separated by 340 nucleotides PS417_07640 and PS417_07645 are separated by 194 nucleotides PS417_07645 and PS417_07650 are separated by 234 nucleotides
PS417_07635: PS417_07635 - transcription-repair coupling factor, at 1,680,634 to 1,684,083
_07635
PS417_07640: PS417_07640 - glyceraldehyde-3-phosphate dehydrogenase, at 1,684,424 to 1,685,887
_07640
PS417_07645: PS417_07645 - hypothetical protein, at 1,686,082 to 1,686,615
_07645
PS417_07650: PS417_07650 - pyridine nucleotide-disulfide oxidoreductase, at 1,686,850 to 1,688,244
_07650
Position (kb)
1684
1685
1686 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1683.471 kb on + strand, within PS417_07635 at 1683.505 kb on - strand, within PS417_07635 at 1683.753 kb on + strand at 1683.753 kb on + strand at 1683.754 kb on - strand at 1683.754 kb on - strand at 1683.756 kb on + strand at 1684.120 kb on - strand at 1684.223 kb on + strand at 1684.224 kb on - strand at 1684.224 kb on - strand at 1684.224 kb on - strand at 1684.310 kb on - strand at 1684.397 kb on - strand at 1684.397 kb on - strand at 1684.421 kb on + strand at 1684.557 kb on - strand at 1684.557 kb on - strand at 1685.081 kb on + strand, within PS417_07640 at 1685.402 kb on + strand, within PS417_07640 at 1685.640 kb on - strand, within PS417_07640 at 1685.649 kb on - strand, within PS417_07640 at 1685.788 kb on - strand at 1685.792 kb on + strand at 1685.885 kb on + strand at 1685.885 kb on + strand at 1685.917 kb on + strand at 1685.935 kb on - strand at 1686.003 kb on + strand at 1686.004 kb on - strand at 1686.258 kb on + strand, within PS417_07645 at 1686.258 kb on + strand, within PS417_07645 at 1686.258 kb on + strand, within PS417_07645 at 1686.259 kb on - strand, within PS417_07645 at 1686.333 kb on + strand, within PS417_07645 at 1686.333 kb on + strand at 1686.334 kb on - strand, within PS417_07645 at 1686.334 kb on - strand, within PS417_07645 at 1686.377 kb on + strand, within PS417_07645 at 1686.377 kb on + strand, within PS417_07645 at 1686.377 kb on + strand, within PS417_07645 at 1686.378 kb on - strand, within PS417_07645 at 1686.378 kb on - strand, within PS417_07645 at 1686.378 kb on - strand, within PS417_07645 at 1686.381 kb on - strand, within PS417_07645 at 1686.446 kb on + strand, within PS417_07645 at 1686.446 kb on + strand, within PS417_07645 at 1686.447 kb on - strand, within PS417_07645 at 1686.447 kb on - strand, within PS417_07645 at 1686.480 kb on - strand, within PS417_07645 at 1686.480 kb on - strand, within PS417_07645 at 1686.590 kb on - strand at 1686.590 kb on - strand at 1686.592 kb on + strand at 1686.615 kb on + strand at 1686.616 kb on - strand at 1686.631 kb on - strand at 1686.631 kb on - strand at 1686.651 kb on + strand at 1686.673 kb on + strand at 1686.694 kb on - strand at 1686.711 kb on + strand at 1686.733 kb on + strand at 1686.733 kb on + strand at 1686.733 kb on + strand at 1686.733 kb on + strand at 1686.735 kb on + strand at 1686.735 kb on + strand at 1686.735 kb on + strand at 1686.736 kb on - strand at 1686.736 kb on - strand at 1686.736 kb on - strand at 1686.736 kb on - strand at 1686.737 kb on + strand at 1686.737 kb on + strand at 1686.738 kb on - strand at 1686.738 kb on - strand at 1686.738 kb on - strand at 1686.738 kb on - strand at 1686.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 1,683,471 + PS417_07635 0.82 -0.8 1,683,505 - PS417_07635 0.83 +0.1 1,683,753 + -0.4 1,683,753 + -1.0 1,683,754 - +1.4 1,683,754 - +0.4 1,683,756 + -0.5 1,684,120 - -0.8 1,684,223 + -1.1 1,684,224 - +1.2 1,684,224 - +0.5 1,684,224 - +2.4 1,684,310 - +0.0 1,684,397 - +1.0 1,684,397 - +0.0 1,684,421 + -2.5 1,684,557 - -0.5 1,684,557 - +0.2 1,685,081 + PS417_07640 0.45 -2.8 1,685,402 + PS417_07640 0.67 +0.5 1,685,640 - PS417_07640 0.83 -1.0 1,685,649 - PS417_07640 0.84 -3.0 1,685,788 - +0.8 1,685,792 + +0.8 1,685,885 + +0.5 1,685,885 + -0.2 1,685,917 + -0.6 1,685,935 - +1.4 1,686,003 + +0.0 1,686,004 - +1.0 1,686,258 + PS417_07645 0.33 +1.6 1,686,258 + PS417_07645 0.33 +0.0 1,686,258 + PS417_07645 0.33 +0.5 1,686,259 - PS417_07645 0.33 -1.2 1,686,333 + PS417_07645 0.47 +0.9 1,686,333 + +2.4 1,686,334 - PS417_07645 0.47 +1.7 1,686,334 - PS417_07645 0.47 +0.0 1,686,377 + PS417_07645 0.55 -1.3 1,686,377 + PS417_07645 0.55 -0.2 1,686,377 + PS417_07645 0.55 -0.8 1,686,378 - PS417_07645 0.55 +0.2 1,686,378 - PS417_07645 0.55 +0.4 1,686,378 - PS417_07645 0.55 +0.6 1,686,381 - PS417_07645 0.56 -2.0 1,686,446 + PS417_07645 0.68 -0.1 1,686,446 + PS417_07645 0.68 +0.0 1,686,447 - PS417_07645 0.68 +0.5 1,686,447 - PS417_07645 0.68 -1.0 1,686,480 - PS417_07645 0.75 +0.2 1,686,480 - PS417_07645 0.75 +0.5 1,686,590 - +0.3 1,686,590 - -0.8 1,686,592 + -0.1 1,686,615 + -1.6 1,686,616 - -2.0 1,686,631 - +0.5 1,686,631 - -0.7 1,686,651 + -0.6 1,686,673 + -0.6 1,686,694 - +0.0 1,686,711 + +0.1 1,686,733 + +0.6 1,686,733 + +0.8 1,686,733 + -0.2 1,686,733 + -2.5 1,686,735 + +1.4 1,686,735 + -0.5 1,686,735 + -0.1 1,686,736 - +0.2 1,686,736 - +0.4 1,686,736 - -1.5 1,686,736 - +0.7 1,686,737 + +0.0 1,686,737 + -0.4 1,686,738 - +0.1 1,686,738 - -0.4 1,686,738 - +0.5 1,686,738 - -0.6 1,686,738 - -1.3
Or see this region's nucleotide sequence