Experiment: GL_4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sbcB and yeeD are separated by 42 nucleotides yeeD and yeeE are separated by 13 nucleotides yeeE and yeeF are separated by 178 nucleotides
b2011: sbcB - exonuclease I (NCBI), at 2,080,780 to 2,082,207
sbcB
b2012: yeeD - hypothetical protein (NCBI), at 2,082,250 to 2,082,477
yeeD
b2013: yeeE - predicted inner membrane protein (NCBI), at 2,082,491 to 2,083,549
yeeE
b2014: yeeF - putative amino acid/amine transport protein (VIMSS), at 2,083,728 to 2,085,086
yeeF
Position (kb)
2082
2083
2084 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2081.513 kb on + strand, within sbcB at 2081.518 kb on - strand, within sbcB at 2081.625 kb on + strand, within sbcB at 2081.675 kb on + strand, within sbcB at 2081.679 kb on + strand, within sbcB at 2081.688 kb on - strand, within sbcB at 2081.701 kb on + strand, within sbcB at 2081.862 kb on - strand, within sbcB at 2081.862 kb on - strand, within sbcB at 2081.885 kb on - strand, within sbcB at 2082.093 kb on + strand at 2082.093 kb on + strand at 2082.168 kb on + strand at 2082.266 kb on + strand at 2082.266 kb on + strand at 2082.266 kb on + strand at 2082.299 kb on + strand, within yeeD at 2082.299 kb on + strand, within yeeD at 2082.429 kb on - strand, within yeeD at 2082.537 kb on - strand at 2082.537 kb on - strand at 2082.544 kb on - strand at 2082.544 kb on - strand at 2082.546 kb on + strand at 2082.674 kb on + strand, within yeeE at 2082.694 kb on - strand, within yeeE at 2082.775 kb on - strand, within yeeE at 2082.775 kb on - strand, within yeeE at 2082.851 kb on - strand, within yeeE at 2082.881 kb on + strand, within yeeE at 2082.897 kb on - strand, within yeeE at 2082.897 kb on - strand, within yeeE at 2082.915 kb on - strand, within yeeE at 2082.973 kb on + strand, within yeeE at 2082.973 kb on + strand, within yeeE at 2082.981 kb on - strand, within yeeE at 2083.040 kb on - strand, within yeeE at 2083.040 kb on - strand, within yeeE at 2083.126 kb on + strand, within yeeE at 2083.139 kb on + strand, within yeeE at 2083.183 kb on + strand, within yeeE at 2083.186 kb on - strand, within yeeE at 2083.186 kb on - strand, within yeeE at 2083.258 kb on - strand, within yeeE at 2083.310 kb on + strand, within yeeE at 2083.319 kb on - strand, within yeeE at 2083.319 kb on - strand, within yeeE at 2083.462 kb on + strand at 2083.470 kb on + strand at 2083.498 kb on - strand at 2083.498 kb on - strand at 2083.498 kb on - strand at 2083.537 kb on - strand at 2083.693 kb on - strand at 2083.807 kb on + strand at 2083.877 kb on - strand, within yeeF at 2083.887 kb on - strand, within yeeF at 2083.939 kb on + strand, within yeeF at 2083.957 kb on + strand, within yeeF at 2083.996 kb on + strand, within yeeF at 2084.160 kb on + strand, within yeeF at 2084.160 kb on + strand, within yeeF at 2084.200 kb on - strand, within yeeF at 2084.206 kb on - strand, within yeeF at 2084.260 kb on + strand, within yeeF at 2084.276 kb on + strand, within yeeF at 2084.284 kb on + strand, within yeeF at 2084.284 kb on + strand, within yeeF at 2084.421 kb on + strand, within yeeF at 2084.421 kb on + strand, within yeeF at 2084.450 kb on - strand, within yeeF at 2084.462 kb on - strand, within yeeF
Per-strain Table
Position Strand Gene LocusTag Fraction GL_4 remove 2,081,513 + sbcB b2011 0.51 -1.0 2,081,518 - sbcB b2011 0.52 +0.2 2,081,625 + sbcB b2011 0.59 -1.6 2,081,675 + sbcB b2011 0.63 +0.5 2,081,679 + sbcB b2011 0.63 -0.5 2,081,688 - sbcB b2011 0.64 +0.4 2,081,701 + sbcB b2011 0.64 -0.6 2,081,862 - sbcB b2011 0.76 -1.0 2,081,862 - sbcB b2011 0.76 +0.7 2,081,885 - sbcB b2011 0.77 -0.3 2,082,093 + -1.4 2,082,093 + -0.1 2,082,168 + +1.6 2,082,266 + +1.5 2,082,266 + -0.6 2,082,266 + +1.6 2,082,299 + yeeD b2012 0.21 -0.5 2,082,299 + yeeD b2012 0.21 -2.1 2,082,429 - yeeD b2012 0.79 +0.5 2,082,537 - +1.6 2,082,537 - -0.6 2,082,544 - +0.9 2,082,544 - -1.7 2,082,546 + -0.2 2,082,674 + yeeE b2013 0.17 -1.0 2,082,694 - yeeE b2013 0.19 +1.1 2,082,775 - yeeE b2013 0.27 -1.4 2,082,775 - yeeE b2013 0.27 +1.5 2,082,851 - yeeE b2013 0.34 +0.1 2,082,881 + yeeE b2013 0.37 +1.2 2,082,897 - yeeE b2013 0.38 +0.5 2,082,897 - yeeE b2013 0.38 -0.9 2,082,915 - yeeE b2013 0.40 -0.4 2,082,973 + yeeE b2013 0.46 -1.1 2,082,973 + yeeE b2013 0.46 +0.6 2,082,981 - yeeE b2013 0.46 +0.4 2,083,040 - yeeE b2013 0.52 +0.6 2,083,040 - yeeE b2013 0.52 -0.2 2,083,126 + yeeE b2013 0.60 +0.5 2,083,139 + yeeE b2013 0.61 -0.1 2,083,183 + yeeE b2013 0.65 -1.6 2,083,186 - yeeE b2013 0.66 -0.3 2,083,186 - yeeE b2013 0.66 +0.1 2,083,258 - yeeE b2013 0.72 +0.2 2,083,310 + yeeE b2013 0.77 +0.9 2,083,319 - yeeE b2013 0.78 +0.1 2,083,319 - yeeE b2013 0.78 -0.1 2,083,462 + +1.1 2,083,470 + +0.6 2,083,498 - +0.5 2,083,498 - -0.5 2,083,498 - -0.2 2,083,537 - -1.6 2,083,693 - -1.1 2,083,807 + +2.5 2,083,877 - yeeF b2014 0.11 +0.7 2,083,887 - yeeF b2014 0.12 +1.1 2,083,939 + yeeF b2014 0.16 +2.1 2,083,957 + yeeF b2014 0.17 +2.5 2,083,996 + yeeF b2014 0.20 -0.7 2,084,160 + yeeF b2014 0.32 -0.8 2,084,160 + yeeF b2014 0.32 -0.1 2,084,200 - yeeF b2014 0.35 +0.8 2,084,206 - yeeF b2014 0.35 +0.6 2,084,260 + yeeF b2014 0.39 +0.4 2,084,276 + yeeF b2014 0.40 +1.0 2,084,284 + yeeF b2014 0.41 +1.5 2,084,284 + yeeF b2014 0.41 +0.9 2,084,421 + yeeF b2014 0.51 -0.5 2,084,421 + yeeF b2014 0.51 -0.3 2,084,450 - yeeF b2014 0.53 +0.9 2,084,462 - yeeF b2014 0.54 -0.4
Or see this region's nucleotide sequence