Strain Fitness in Escherichia coli BL21 around ECD_02250

Experiment: Control_TnD

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02249 and ECD_02250 are separated by 120 nucleotidesECD_02250 and ECD_02251 are separated by 33 nucleotidesECD_02251 and ECD_02252 overlap by 1 nucleotides ECD_02249: ECD_02249 - fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme, at 2,329,017 to 2,331,023 _02249 ECD_02250: ECD_02250 - uncharacterized protein, at 2,331,144 to 2,331,422 _02250 ECD_02251: ECD_02251 - Elongation Factor P Lys34 hydroxylase, at 2,331,456 to 2,332,004 _02251 ECD_02252: ECD_02252 - TauE/TSUP family inner membrane protein, at 2,332,004 to 2,332,813 _02252 Position (kb) 2331 2332Strain fitness (log2 ratio) -2 -1 0 1at 2330.210 kb on + strand, within ECD_02249at 2330.211 kb on - strand, within ECD_02249at 2330.383 kb on + strand, within ECD_02249at 2330.565 kb on - strand, within ECD_02249at 2330.846 kb on + strandat 2330.860 kb on + strandat 2331.073 kb on + strandat 2331.073 kb on + strandat 2331.074 kb on - strandat 2331.261 kb on - strand, within ECD_02250at 2331.512 kb on - strand, within ECD_02251at 2331.515 kb on - strand, within ECD_02251at 2331.572 kb on - strand, within ECD_02251at 2331.592 kb on - strand, within ECD_02251at 2331.880 kb on - strand, within ECD_02251at 2331.902 kb on - strand, within ECD_02251at 2331.939 kb on - strand, within ECD_02251at 2331.963 kb on - strandat 2332.005 kb on + strandat 2332.006 kb on - strandat 2332.006 kb on - strandat 2332.056 kb on - strandat 2332.395 kb on + strand, within ECD_02252

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnD
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2,330,210 + ECD_02249 0.59 -0.9
2,330,211 - ECD_02249 0.59 +0.6
2,330,383 + ECD_02249 0.68 +0.8
2,330,565 - ECD_02249 0.77 +0.5
2,330,846 + -0.3
2,330,860 + -0.4
2,331,073 + +0.6
2,331,073 + +0.4
2,331,074 - -0.7
2,331,261 - ECD_02250 0.42 -1.0
2,331,512 - ECD_02251 0.10 -0.3
2,331,515 - ECD_02251 0.11 -1.5
2,331,572 - ECD_02251 0.21 +1.7
2,331,592 - ECD_02251 0.25 -0.2
2,331,880 - ECD_02251 0.77 -2.0
2,331,902 - ECD_02251 0.81 +0.3
2,331,939 - ECD_02251 0.88 +0.5
2,331,963 - -1.5
2,332,005 + -0.3
2,332,006 - +0.3
2,332,006 - -0.3
2,332,056 - -1.3
2,332,395 + ECD_02252 0.48 -0.6

Or see this region's nucleotide sequence