Strain Fitness in Escherichia coli BL21 around ECD_00312

Experiment: Control_TnD

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00310 and ECD_00311 are separated by 34 nucleotidesECD_00311 and ECD_00312 are separated by 185 nucleotidesECD_00312 and ECD_00313 are separated by 81 nucleotidesECD_00313 and ECD_00314 overlap by 82 nucleotidesECD_00314 and ECD_00315 are separated by 163 nucleotides ECD_00310: ECD_00310 - alcohol dehydrogenase class III; glutathione-dependent formaldehyde dehydrogenase, at 347,798 to 348,907 _00310 ECD_00311: ECD_00311 - regulator protein that represses frmRAB operon, at 348,942 to 349,217 _00311 ECD_00312: ECD_00312 - outer membrane protein, at 349,403 to 350,176 _00312 ECD_00313: ECD_00313 - IS1 protein InsB, at 350,258 to 350,761 _00313 ECD_00314: ECD_00314 - IS1 protein InsA, at 350,680 to 350,955 _00314 ECD_00315: ECD_00315 - taurine ABC transporter periplasmic binding protein, at 351,119 to 352,081 _00315 Position (kb) 349 350 351Strain fitness (log2 ratio) -1 0 1at 348.477 kb on + strand, within ECD_00310at 348.478 kb on - strand, within ECD_00310at 348.505 kb on - strand, within ECD_00310at 348.510 kb on + strand, within ECD_00310at 348.645 kb on + strand, within ECD_00310at 348.773 kb on - strand, within ECD_00310at 348.773 kb on - strand, within ECD_00310at 348.811 kb on + strandat 349.007 kb on - strand, within ECD_00311at 349.105 kb on - strand, within ECD_00311at 349.246 kb on - strandat 349.288 kb on - strandat 349.291 kb on - strandat 349.445 kb on - strandat 349.445 kb on - strandat 349.498 kb on + strand, within ECD_00312at 349.499 kb on - strand, within ECD_00312at 349.610 kb on + strand, within ECD_00312at 349.611 kb on - strand, within ECD_00312at 349.618 kb on + strand, within ECD_00312at 349.666 kb on + strand, within ECD_00312at 349.667 kb on - strand, within ECD_00312at 349.668 kb on + strand, within ECD_00312at 349.669 kb on - strand, within ECD_00312at 349.672 kb on + strand, within ECD_00312at 349.673 kb on - strand, within ECD_00312at 349.705 kb on - strand, within ECD_00312at 349.732 kb on + strand, within ECD_00312at 349.735 kb on - strand, within ECD_00312at 349.735 kb on - strand, within ECD_00312at 349.742 kb on + strand, within ECD_00312at 349.779 kb on + strand, within ECD_00312at 349.783 kb on - strand, within ECD_00312at 349.841 kb on - strand, within ECD_00312at 349.877 kb on - strand, within ECD_00312at 349.928 kb on - strand, within ECD_00312at 350.046 kb on + strand, within ECD_00312at 350.046 kb on + strand, within ECD_00312at 350.047 kb on - strand, within ECD_00312at 350.234 kb on + strandat 351.073 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_TnD
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348,477 + ECD_00310 0.61 +0.1
348,478 - ECD_00310 0.61 -0.1
348,505 - ECD_00310 0.64 -0.5
348,510 + ECD_00310 0.64 +0.1
348,645 + ECD_00310 0.76 -0.1
348,773 - ECD_00310 0.88 -0.0
348,773 - ECD_00310 0.88 -0.5
348,811 + +0.2
349,007 - ECD_00311 0.24 +0.3
349,105 - ECD_00311 0.59 +0.0
349,246 - +0.9
349,288 - +0.6
349,291 - -0.1
349,445 - +0.5
349,445 - +0.9
349,498 + ECD_00312 0.12 +0.1
349,499 - ECD_00312 0.12 +0.7
349,610 + ECD_00312 0.27 +0.4
349,611 - ECD_00312 0.27 +0.9
349,618 + ECD_00312 0.28 -0.2
349,666 + ECD_00312 0.34 +0.4
349,667 - ECD_00312 0.34 +0.2
349,668 + ECD_00312 0.34 +0.4
349,669 - ECD_00312 0.34 +0.1
349,672 + ECD_00312 0.35 -0.6
349,673 - ECD_00312 0.35 +0.5
349,705 - ECD_00312 0.39 -0.1
349,732 + ECD_00312 0.43 -0.2
349,735 - ECD_00312 0.43 +0.3
349,735 - ECD_00312 0.43 +0.5
349,742 + ECD_00312 0.44 +0.5
349,779 + ECD_00312 0.49 +1.2
349,783 - ECD_00312 0.49 -0.7
349,841 - ECD_00312 0.57 +0.4
349,877 - ECD_00312 0.61 +0.1
349,928 - ECD_00312 0.68 +0.9
350,046 + ECD_00312 0.83 +0.3
350,046 + ECD_00312 0.83 +0.2
350,047 - ECD_00312 0.83 -0.2
350,234 + +0.4
351,073 + -0.6

Or see this region's nucleotide sequence