Experiment: NLDM_defined with MS_media 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS00975 and ABID97_RS00980 are separated by 10 nucleotides ABID97_RS00980 and ABID97_RS00985 are separated by 146 nucleotides ABID97_RS00985 and ABID97_RS00990 overlap by 4 nucleotides ABID97_RS00990 and ABID97_RS00995 overlap by 4 nucleotides
ABID97_RS00975: ABID97_RS00975 - winged helix DNA-binding domain-containing protein, at 196,823 to 197,926
_RS00975
ABID97_RS00980: ABID97_RS00980 - pseudouridine synthase, at 197,937 to 198,761
_RS00980
ABID97_RS00985: ABID97_RS00985 - glycosyltransferase, at 198,908 to 200,047
_RS00985
ABID97_RS00990: ABID97_RS00990 - hypothetical protein, at 200,044 to 200,538
_RS00990
ABID97_RS00995: ABID97_RS00995 - tyrosine-protein phosphatase, at 200,535 to 201,065
_RS00995
Position (kb)
198
199
200
201 Strain fitness (log2 ratio)
-2
-1
0
1 at 198.284 kb on - strand, within ABID97_RS00980 at 198.530 kb on - strand, within ABID97_RS00980 at 199.058 kb on + strand, within ABID97_RS00985 at 199.058 kb on + strand at 199.059 kb on - strand, within ABID97_RS00985 at 199.070 kb on + strand, within ABID97_RS00985 at 199.071 kb on - strand, within ABID97_RS00985 at 199.071 kb on - strand, within ABID97_RS00985 at 199.110 kb on - strand, within ABID97_RS00985 at 199.902 kb on - strand, within ABID97_RS00985 at 199.922 kb on + strand, within ABID97_RS00985 at 199.923 kb on - strand, within ABID97_RS00985 at 200.749 kb on + strand, within ABID97_RS00995 at 200.749 kb on + strand, within ABID97_RS00995 at 200.992 kb on - strand, within ABID97_RS00995
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with MS_media 0.5 X remove 198,284 - ABID97_RS00980 0.42 -0.3 198,530 - ABID97_RS00980 0.72 +0.1 199,058 + ABID97_RS00985 0.13 -0.8 199,058 + +0.2 199,059 - ABID97_RS00985 0.13 -0.3 199,070 + ABID97_RS00985 0.14 +0.8 199,071 - ABID97_RS00985 0.14 +0.0 199,071 - ABID97_RS00985 0.14 -0.6 199,110 - ABID97_RS00985 0.18 -2.6 199,902 - ABID97_RS00985 0.87 -0.7 199,922 + ABID97_RS00985 0.89 -0.2 199,923 - ABID97_RS00985 0.89 -0.5 200,749 + ABID97_RS00995 0.40 -0.2 200,749 + ABID97_RS00995 0.40 -0.6 200,992 - ABID97_RS00995 0.86 -0.1
Or see this region's nucleotide sequence