Experiment: NLDM_defined with ATCC Wolfe's mineral mix 20 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS12655 and ABID97_RS12660 overlap by 1 nucleotides ABID97_RS12660 and ABID97_RS12665 overlap by 4 nucleotides ABID97_RS12665 and ABID97_RS12670 are separated by 31 nucleotides ABID97_RS12670 and ABID97_RS12675 are separated by 2 nucleotides
ABID97_RS12655: ABID97_RS12655 - formylmethanofuran dehydrogenase subunit A, at 2,626,854 to 2,628,539
_RS12655
ABID97_RS12660: ABID97_RS12660 - formylmethanofuran--tetrahydromethanopterin N-formyltransferase, at 2,628,539 to 2,629,504
_RS12660
ABID97_RS12665: ABID97_RS12665 - formylmethanofuran dehydrogenase subunit C, at 2,629,501 to 2,630,328
_RS12665
ABID97_RS12670: ABID97_RS12670 - formaldehyde-activating enzyme, at 2,630,360 to 2,630,893
_RS12670
ABID97_RS12675: ABID97_RS12675 - aminodeoxychorismate synthase component I, at 2,630,896 to 2,632,813
_RS12675
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-2
-1
0
1 at 2628.981 kb on - strand, within ABID97_RS12660 at 2629.434 kb on - strand at 2629.434 kb on - strand at 2630.049 kb on + strand, within ABID97_RS12665 at 2630.695 kb on + strand, within ABID97_RS12670 at 2630.696 kb on - strand, within ABID97_RS12670 at 2630.771 kb on + strand, within ABID97_RS12670 at 2630.771 kb on + strand, within ABID97_RS12670 at 2630.772 kb on - strand, within ABID97_RS12670 at 2630.772 kb on - strand, within ABID97_RS12670
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with ATCC Wolfe's mineral mix 20 X remove 2,628,981 - ABID97_RS12660 0.46 +0.0 2,629,434 - -2.4 2,629,434 - -1.1 2,630,049 + ABID97_RS12665 0.66 -1.2 2,630,695 + ABID97_RS12670 0.63 -0.6 2,630,696 - ABID97_RS12670 0.63 +0.2 2,630,771 + ABID97_RS12670 0.77 -1.1 2,630,771 + ABID97_RS12670 0.77 -0.9 2,630,772 - ABID97_RS12670 0.77 -0.9 2,630,772 - ABID97_RS12670 0.77 -1.5
Or see this region's nucleotide sequence