Experiment: NLDM_defined with ATCC Wolfe's mineral mix 10 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS10875 and ABID97_RS10880 are separated by 136 nucleotides ABID97_RS10880 and ABID97_RS10885 are separated by 100 nucleotides ABID97_RS10885 and ABID97_RS10890 are separated by 147 nucleotides
ABID97_RS10875: ABID97_RS10875 - peroxiredoxin, at 2,252,737 to 2,253,213
_RS10875
ABID97_RS10880: ABID97_RS10880 - Mth938-like domain-containing protein, at 2,253,350 to 2,253,733
_RS10880
ABID97_RS10885: ABID97_RS10885 - pyridoxal phosphate-dependent aminotransferase, at 2,253,834 to 2,255,066
_RS10885
ABID97_RS10890: ABID97_RS10890 - homoserine dehydrogenase, at 2,255,214 to 2,256,536
_RS10890
Position (kb)
2253
2254
2255
2256 Strain fitness (log2 ratio)
-2
-1
0
1 at 2253.420 kb on + strand, within ABID97_RS10880 at 2253.420 kb on + strand, within ABID97_RS10880 at 2253.421 kb on - strand, within ABID97_RS10880 at 2253.421 kb on - strand, within ABID97_RS10880 at 2253.760 kb on + strand at 2254.128 kb on + strand, within ABID97_RS10885 at 2254.461 kb on + strand, within ABID97_RS10885 at 2254.476 kb on + strand, within ABID97_RS10885 at 2254.557 kb on + strand, within ABID97_RS10885 at 2254.830 kb on + strand, within ABID97_RS10885 at 2254.830 kb on + strand, within ABID97_RS10885 at 2254.860 kb on + strand, within ABID97_RS10885 at 2255.231 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with ATCC Wolfe's mineral mix 10 X remove 2,253,420 + ABID97_RS10880 0.18 -1.7 2,253,420 + ABID97_RS10880 0.18 +0.3 2,253,421 - ABID97_RS10880 0.18 -1.9 2,253,421 - ABID97_RS10880 0.18 -0.2 2,253,760 + -1.4 2,254,128 + ABID97_RS10885 0.24 +0.1 2,254,461 + ABID97_RS10885 0.51 -1.8 2,254,476 + ABID97_RS10885 0.52 -0.8 2,254,557 + ABID97_RS10885 0.59 -1.7 2,254,830 + ABID97_RS10885 0.81 -1.9 2,254,830 + ABID97_RS10885 0.81 -1.8 2,254,860 + ABID97_RS10885 0.83 -0.8 2,255,231 - -0.5
Or see this region's nucleotide sequence