Experiment: NLDM_defined with PEG 6000 15 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS26105 and ABID97_RS26110 overlap by 4 nucleotides ABID97_RS26110 and ABID97_RS26115 are separated by 108 nucleotides ABID97_RS26115 and ABID97_RS26120 are separated by 51 nucleotides
ABID97_RS26105: ABID97_RS26105 - sterol desaturase family protein, at 145,048 to 145,953
_RS26105
ABID97_RS26110: ABID97_RS26110 - coniferyl aldehyde dehydrogenase, at 145,950 to 147,401
_RS26110
ABID97_RS26115: ABID97_RS26115 - hypothetical protein, at 147,510 to 147,854
_RS26115
ABID97_RS26120: ABID97_RS26120 - hypothetical protein, at 147,906 to 149,237
_RS26120
Position (kb) 145
146
147
148 Strain fitness (log2 ratio) -1
0
1 at 145.399 kb on + strand, within ABID97_RS26105 at 145.514 kb on - strand, within ABID97_RS26105 at 145.690 kb on + strand, within ABID97_RS26105 at 145.691 kb on - strand, within ABID97_RS26105 at 145.831 kb on + strand, within ABID97_RS26105 at 147.049 kb on - strand, within ABID97_RS26110 at 147.108 kb on + strand, within ABID97_RS26110 at 147.252 kb on + strand, within ABID97_RS26110 at 147.253 kb on - strand, within ABID97_RS26110 at 147.382 kb on - strand at 147.382 kb on - strand at 147.853 kb on - strand at 147.995 kb on - strand at 148.316 kb on - strand, within ABID97_RS26120
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with PEG 6000 15 vol% remove 145,399 + ABID97_RS26105 0.39 +0.0 145,514 - ABID97_RS26105 0.51 -0.5 145,690 + ABID97_RS26105 0.71 +0.0 145,691 - ABID97_RS26105 0.71 -0.0 145,831 + ABID97_RS26105 0.86 +1.2 147,049 - ABID97_RS26110 0.76 -0.7 147,108 + ABID97_RS26110 0.80 -0.5 147,252 + ABID97_RS26110 0.90 -0.9 147,253 - ABID97_RS26110 0.90 +0.2 147,382 - -0.5 147,382 - -0.1 147,853 - -0.4 147,995 - +0.0 148,316 - ABID97_RS26120 0.31 -0.3
Or see this region's nucleotide sequence