Experiment: NLDM_defined with PEG 6000 20 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS00975 and ABID97_RS00980 are separated by 10 nucleotides ABID97_RS00980 and ABID97_RS00985 are separated by 146 nucleotides ABID97_RS00985 and ABID97_RS00990 overlap by 4 nucleotides ABID97_RS00990 and ABID97_RS00995 overlap by 4 nucleotides
ABID97_RS00975: ABID97_RS00975 - winged helix DNA-binding domain-containing protein, at 196,823 to 197,926
_RS00975
ABID97_RS00980: ABID97_RS00980 - pseudouridine synthase, at 197,937 to 198,761
_RS00980
ABID97_RS00985: ABID97_RS00985 - glycosyltransferase, at 198,908 to 200,047
_RS00985
ABID97_RS00990: ABID97_RS00990 - hypothetical protein, at 200,044 to 200,538
_RS00990
ABID97_RS00995: ABID97_RS00995 - tyrosine-protein phosphatase, at 200,535 to 201,065
_RS00995
Position (kb) 198
199
200
201 Strain fitness (log2 ratio) -1
0
1 at 198.284 kb on - strand, within ABID97_RS00980 at 198.530 kb on - strand, within ABID97_RS00980 at 199.058 kb on + strand, within ABID97_RS00985 at 199.058 kb on + strand at 199.059 kb on - strand, within ABID97_RS00985 at 199.070 kb on + strand, within ABID97_RS00985 at 199.071 kb on - strand, within ABID97_RS00985 at 199.071 kb on - strand, within ABID97_RS00985 at 199.110 kb on - strand, within ABID97_RS00985 at 199.902 kb on - strand, within ABID97_RS00985 at 199.922 kb on + strand, within ABID97_RS00985 at 199.923 kb on - strand, within ABID97_RS00985 at 200.749 kb on + strand, within ABID97_RS00995 at 200.749 kb on + strand, within ABID97_RS00995 at 200.992 kb on - strand, within ABID97_RS00995
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with PEG 6000 20 vol% remove 198,284 - ABID97_RS00980 0.42 -0.0 198,530 - ABID97_RS00980 0.72 +0.6 199,058 + ABID97_RS00985 0.13 +0.4 199,058 + -0.9 199,059 - ABID97_RS00985 0.13 -1.7 199,070 + ABID97_RS00985 0.14 -1.1 199,071 - ABID97_RS00985 0.14 -0.2 199,071 - ABID97_RS00985 0.14 -0.2 199,110 - ABID97_RS00985 0.18 -0.9 199,902 - ABID97_RS00985 0.87 -0.7 199,922 + ABID97_RS00985 0.89 -0.5 199,923 - ABID97_RS00985 0.89 -0.2 200,749 + ABID97_RS00995 0.40 +0.4 200,749 + ABID97_RS00995 0.40 -0.3 200,992 - ABID97_RS00995 0.86 +0.2
Or see this region's nucleotide sequence