Experiment: NLDM_defined with PEG 6000 25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS26425 and ABID97_RS26430 are separated by 31 nucleotides ABID97_RS26430 and ABID97_RS26435 overlap by 11 nucleotides ABID97_RS26435 and ABID97_RS26440 are separated by 45 nucleotides
ABID97_RS26425: ABID97_RS26425 - ABC transporter ATP-binding protein, at 204,129 to 204,629
_RS26425
ABID97_RS26430: ABID97_RS26430 - SDR family oxidoreductase, at 204,661 to 205,362
_RS26430
ABID97_RS26435: ABID97_RS26435 - tripartite tricarboxylate transporter substrate binding protein, at 205,352 to 206,323
_RS26435
ABID97_RS26440: ABID97_RS26440 - dihydrodipicolinate synthase family protein, at 206,369 to 207,307
_RS26440
Position (kb) 205
206
207 Strain fitness (log2 ratio) -2
-1
0
1 at 204.438 kb on + strand, within ABID97_RS26425 at 204.439 kb on - strand, within ABID97_RS26425 at 204.465 kb on + strand, within ABID97_RS26425 at 204.465 kb on + strand, within ABID97_RS26425 at 204.554 kb on + strand, within ABID97_RS26425 at 205.576 kb on - strand, within ABID97_RS26435 at 205.767 kb on + strand, within ABID97_RS26435 at 205.768 kb on - strand, within ABID97_RS26435 at 205.884 kb on + strand, within ABID97_RS26435 at 206.055 kb on + strand, within ABID97_RS26435 at 206.485 kb on - strand, within ABID97_RS26440
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_defined with PEG 6000 25 vol% remove 204,438 + ABID97_RS26425 0.62 +0.7 204,439 - ABID97_RS26425 0.62 -0.2 204,465 + ABID97_RS26425 0.67 -2.5 204,465 + ABID97_RS26425 0.67 -0.3 204,554 + ABID97_RS26425 0.85 +0.2 205,576 - ABID97_RS26435 0.23 +0.1 205,767 + ABID97_RS26435 0.43 +0.2 205,768 - ABID97_RS26435 0.43 -0.3 205,884 + ABID97_RS26435 0.55 -1.3 206,055 + ABID97_RS26435 0.72 -0.2 206,485 - ABID97_RS26440 0.12 +1.6
Or see this region's nucleotide sequence