Strain Fitness in Escherichia coli BL21 around ECD_r00010

Experiment: CM1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_r00009 and ECD_t00060 are separated by 183 nucleotidesECD_t00060 and ECD_t00061 are separated by 42 nucleotidesECD_t00061 and ECD_r00010 are separated by 68 nucleotidesECD_r00010 and ECD_03130 are separated by 473 nucleotides ECD_r00009: ECD_r00009 - 23S ribosomal RNA, at 3,285,037 to 3,287,944 _r00009 ECD_t00060: ECD_t00060 - tRNA-Ala, at 3,288,128 to 3,288,203 _t00060 ECD_t00061: ECD_t00061 - tRNA-Ile, at 3,288,246 to 3,288,322 _t00061 ECD_r00010: ECD_r00010 - 16S ribosomal RNA, at 3,288,391 to 3,289,932 _r00010 ECD_03130: ECD_03130 - bacterial transferase hexapeptide domain protein, at 3,290,406 to 3,290,960 _03130 Position (kb) 3288 3289 3290Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3290.231 kb on - strandat 3290.231 kb on - strandat 3290.338 kb on + strandat 3290.339 kb on - strandat 3290.390 kb on + strandat 3290.405 kb on + strandat 3290.405 kb on + strandat 3290.405 kb on + strandat 3290.405 kb on + strandat 3290.406 kb on - strandat 3290.419 kb on + strandat 3290.551 kb on + strand, within ECD_03130at 3290.551 kb on + strand, within ECD_03130at 3290.551 kb on + strand, within ECD_03130at 3290.551 kb on + strand, within ECD_03130at 3290.552 kb on - strand, within ECD_03130at 3290.552 kb on - strand, within ECD_03130at 3290.552 kb on - strand, within ECD_03130at 3290.552 kb on - strand, within ECD_03130at 3290.552 kb on - strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.556 kb on + strand, within ECD_03130at 3290.557 kb on - strand, within ECD_03130at 3290.557 kb on - strand, within ECD_03130at 3290.557 kb on - strand, within ECD_03130at 3290.557 kb on - strand, within ECD_03130at 3290.560 kb on + strand, within ECD_03130at 3290.560 kb on + strand, within ECD_03130at 3290.597 kb on + strand, within ECD_03130at 3290.597 kb on + strand, within ECD_03130at 3290.598 kb on - strand, within ECD_03130at 3290.616 kb on - strand, within ECD_03130at 3290.626 kb on - strand, within ECD_03130at 3290.626 kb on - strand, within ECD_03130at 3290.756 kb on - strand, within ECD_03130at 3290.758 kb on + strand, within ECD_03130at 3290.758 kb on + strand, within ECD_03130at 3290.759 kb on - strand, within ECD_03130at 3290.759 kb on - strand, within ECD_03130at 3290.786 kb on + strand, within ECD_03130at 3290.829 kb on + strand, within ECD_03130at 3290.830 kb on - strand, within ECD_03130at 3290.892 kb on + strand, within ECD_03130at 3290.907 kb on + strandat 3290.908 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction CM1
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3,290,231 - +0.6
3,290,231 - -0.4
3,290,338 + -0.6
3,290,339 - +0.5
3,290,390 + -0.2
3,290,405 + -0.1
3,290,405 + -0.4
3,290,405 + +0.2
3,290,405 + -0.9
3,290,406 - -0.1
3,290,419 + -1.1
3,290,551 + ECD_03130 0.26 +0.1
3,290,551 + ECD_03130 0.26 -0.0
3,290,551 + ECD_03130 0.26 -0.9
3,290,551 + ECD_03130 0.26 -0.4
3,290,552 - ECD_03130 0.26 -1.3
3,290,552 - ECD_03130 0.26 -0.2
3,290,552 - ECD_03130 0.26 -1.2
3,290,552 - ECD_03130 0.26 +0.2
3,290,552 - ECD_03130 0.26 -0.9
3,290,556 + ECD_03130 0.27 -2.3
3,290,556 + ECD_03130 0.27 -0.0
3,290,556 + ECD_03130 0.27 +4.7
3,290,556 + ECD_03130 0.27 -1.6
3,290,556 + ECD_03130 0.27 +0.7
3,290,556 + ECD_03130 0.27 -0.0
3,290,557 - ECD_03130 0.27 +0.5
3,290,557 - ECD_03130 0.27 +0.1
3,290,557 - ECD_03130 0.27 +0.6
3,290,557 - ECD_03130 0.27 +0.4
3,290,560 + ECD_03130 0.28 -0.9
3,290,560 + ECD_03130 0.28 -1.0
3,290,597 + ECD_03130 0.34 +0.6
3,290,597 + ECD_03130 0.34 -0.3
3,290,598 - ECD_03130 0.35 +0.6
3,290,616 - ECD_03130 0.38 +0.7
3,290,626 - ECD_03130 0.40 +0.4
3,290,626 - ECD_03130 0.40 +0.4
3,290,756 - ECD_03130 0.63 +0.9
3,290,758 + ECD_03130 0.63 +0.2
3,290,758 + ECD_03130 0.63 +0.2
3,290,759 - ECD_03130 0.64 -1.5
3,290,759 - ECD_03130 0.64 -1.1
3,290,786 + ECD_03130 0.68 +0.1
3,290,829 + ECD_03130 0.76 +0.3
3,290,830 - ECD_03130 0.76 -0.8
3,290,892 + ECD_03130 0.88 -1.2
3,290,907 + +0.3
3,290,908 - -0.1

Or see this region's nucleotide sequence