Strain Fitness in Escherichia coli BL21 around ECD_02225

Experiment: N4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4
2,305,543	-		ECD_02223	0.79	+0.5
2,305,543	-		ECD_02223	0.79	+1.2
2,305,804	-				+0.3
2,305,806	+				+0.3
2,306,100	-		ECD_02224	0.39	-0.3
2,306,208	+		ECD_02224	0.59	+0.3
2,306,309	-		ECD_02224	0.78	+1.2
2,306,309	-		ECD_02224	0.78	-0.4
2,306,406	-				-0.1
2,306,517	-				+0.3
2,306,517	-				-0.8
2,306,517	-				+0.8
2,306,539	-				+0.4
2,306,539	-				+0.8
2,306,547	+		ECD_02225	0.11	-0.1
2,306,548	-		ECD_02225	0.11	-0.0
2,306,548	-		ECD_02225	0.11	-0.8
2,306,548	-		ECD_02225	0.11	+1.0
2,306,548	-		ECD_02225	0.11	+0.6
2,306,548	-		ECD_02225	0.11	+1.0
2,306,846	+		ECD_02225	0.65	+1.1
2,306,846	+		ECD_02225	0.65	-0.2
2,306,846	+		ECD_02225	0.65	+0.0
2,306,846	+		ECD_02225	0.65	+1.2
2,306,847	-		ECD_02225	0.65	+0.7
2,306,847	-		ECD_02225	0.65	-0.4
2,306,847	-		ECD_02225	0.65	+0.4
2,306,847	-		ECD_02225	0.65	+0.4
2,306,847	-		ECD_02225	0.65	-0.5
2,306,847	-		ECD_02225	0.65	-0.8
2,306,847	-		ECD_02225	0.65	+0.2
2,307,068	+				-0.2
2,307,069	-				-0.1
2,307,069	-				+0.1
2,307,069	-				+1.4
2,307,069	-				+0.3
2,307,069	-				-0.2
2,307,069	-				-0.2
2,307,164	+		ECD_02226	0.11	-0.1
2,307,200	-		ECD_02226	0.16	-0.4
2,307,468	+		ECD_02226	0.58	-0.9
2,307,469	-		ECD_02226	0.58	-1.0
2,307,687	-				-0.0
2,307,687	-				+0.9
2,307,739	+				-0.4
2,307,740	-				+0.9
2,307,740	-				+0.9
2,307,775	+				+0.1
2,307,816	+				+0.4
2,307,816	+				+0.6
2,307,817	-				-0.8
2,307,856	+				-0.1