Strain Fitness in Escherichia coli BL21 around ECD_01005

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01004 and ECD_01005 are separated by 282 nucleotidesECD_01005 and ECD_01006 are separated by 37 nucleotidesECD_01006 and ECD_01007 are separated by 20 nucleotides ECD_01004: ECD_01004 - uncharacterized protein, at 1,068,324 to 1,069,583 _01004 ECD_01005: ECD_01005 - glucose-1-phosphatase/inositol phosphatase, at 1,069,866 to 1,071,107 _01005 ECD_01006: ECD_01006 - uncharacterized protein, at 1,071,145 to 1,071,372 _01006 ECD_01007: ECD_01007 - NAD(P)H:quinone oxidoreductase, at 1,071,393 to 1,071,989 _01007 Position (kb) 1069 1070 1071 1072Strain fitness (log2 ratio) -2 -1 0 1at 1069.880 kb on - strandat 1070.106 kb on + strand, within ECD_01005at 1070.118 kb on + strand, within ECD_01005at 1070.152 kb on + strand, within ECD_01005at 1070.186 kb on - strand, within ECD_01005at 1070.186 kb on - strand, within ECD_01005at 1070.193 kb on + strand, within ECD_01005at 1070.433 kb on + strand, within ECD_01005at 1070.584 kb on - strand, within ECD_01005at 1070.946 kb on + strand, within ECD_01005at 1070.946 kb on + strand, within ECD_01005at 1070.975 kb on + strand, within ECD_01005at 1071.194 kb on - strand, within ECD_01006at 1071.194 kb on - strand, within ECD_01006at 1071.580 kb on - strand, within ECD_01007at 1071.580 kb on - strand, within ECD_01007at 1071.580 kb on - strand, within ECD_01007at 1071.618 kb on - strand, within ECD_01007at 1071.621 kb on - strand, within ECD_01007at 1071.679 kb on + strand, within ECD_01007at 1072.010 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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1,069,880 - +0.6
1,070,106 + ECD_01005 0.19 +0.1
1,070,118 + ECD_01005 0.20 -0.4
1,070,152 + ECD_01005 0.23 +0.7
1,070,186 - ECD_01005 0.26 +0.3
1,070,186 - ECD_01005 0.26 -0.3
1,070,193 + ECD_01005 0.26 -0.4
1,070,433 + ECD_01005 0.46 +0.9
1,070,584 - ECD_01005 0.58 +0.9
1,070,946 + ECD_01005 0.87 +0.2
1,070,946 + ECD_01005 0.87 +1.0
1,070,975 + ECD_01005 0.89 -0.2
1,071,194 - ECD_01006 0.21 -0.8
1,071,194 - ECD_01006 0.21 -2.6
1,071,580 - ECD_01007 0.31 +0.0
1,071,580 - ECD_01007 0.31 -0.4
1,071,580 - ECD_01007 0.31 +0.4
1,071,618 - ECD_01007 0.38 -0.5
1,071,621 - ECD_01007 0.38 -0.3
1,071,679 + ECD_01007 0.48 -0.5
1,072,010 - +0.7

Or see this region's nucleotide sequence