Strain Fitness in Escherichia coli BL21 around ECD_00600

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00599 and ECD_00600 are separated by 100 nucleotidesECD_00600 and ECD_00601 are separated by 109 nucleotides ECD_00599: ECD_00599 - octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase, at 621,999 to 622,640 _00599 ECD_00600: ECD_00600 - UPF0250 family protein, at 622,741 to 623,004 _00600 ECD_00601: ECD_00601 - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5), at 623,114 to 624,325 _00601 Position (kb) 622 623 624Strain fitness (log2 ratio) -1 0 1at 622.683 kb on - strandat 622.685 kb on - strandat 622.785 kb on - strand, within ECD_00600at 623.028 kb on - strandat 623.032 kb on - strandat 623.032 kb on - strandat 623.073 kb on - strandat 623.073 kb on - strandat 623.073 kb on - strandat 623.073 kb on - strandat 623.305 kb on - strand, within ECD_00601at 623.305 kb on - strand, within ECD_00601at 623.305 kb on - strand, within ECD_00601at 623.305 kb on - strand, within ECD_00601at 623.305 kb on - strand, within ECD_00601at 623.305 kb on - strand, within ECD_00601at 623.349 kb on + strand, within ECD_00601at 623.350 kb on - strand, within ECD_00601at 623.712 kb on - strand, within ECD_00601at 623.763 kb on + strand, within ECD_00601at 623.790 kb on - strand, within ECD_00601at 623.790 kb on - strand, within ECD_00601at 623.833 kb on - strand, within ECD_00601at 623.833 kb on - strand, within ECD_00601at 623.833 kb on - strand, within ECD_00601at 623.923 kb on + strand, within ECD_00601

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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622,683 - +0.9
622,685 - -0.3
622,785 - ECD_00600 0.17 +1.5
623,028 - -0.5
623,032 - +0.7
623,032 - -0.3
623,073 - +0.2
623,073 - -0.8
623,073 - -0.1
623,073 - +0.2
623,305 - ECD_00601 0.16 +0.7
623,305 - ECD_00601 0.16 +1.5
623,305 - ECD_00601 0.16 +0.3
623,305 - ECD_00601 0.16 +0.2
623,305 - ECD_00601 0.16 +0.3
623,305 - ECD_00601 0.16 -0.7
623,349 + ECD_00601 0.19 +1.1
623,350 - ECD_00601 0.19 -0.1
623,712 - ECD_00601 0.49 +0.3
623,763 + ECD_00601 0.54 +1.4
623,790 - ECD_00601 0.56 +1.3
623,790 - ECD_00601 0.56 +0.1
623,833 - ECD_00601 0.59 +0.3
623,833 - ECD_00601 0.59 -0.2
623,833 - ECD_00601 0.59 +1.1
623,923 + ECD_00601 0.67 +0.5

Or see this region's nucleotide sequence