Strain Fitness in Escherichia coli BL21 around ECD_00196

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00194 and ECD_00195 overlap by 4 nucleotidesECD_00195 and ECD_00196 are separated by 117 nucleotidesECD_00196 and ECD_00197 are separated by 39 nucleotidesECD_00197 and ECD_00198 overlap by 8 nucleotides ECD_00194: ECD_00194 - tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase, SAM-dependent, at 221,728 to 222,435 _00194 ECD_00195: ECD_00195 - putative outer membrane protein, at 222,432 to 222,836 _00195 ECD_00196: ECD_00196 - DL-methionine transporter subunit, at 222,954 to 223,769 _00196 ECD_00197: ECD_00197 - DL-methionine transporter subunit, at 223,809 to 224,462 _00197 ECD_00198: ECD_00198 - DL-methionine transporter subunit, at 224,455 to 225,486 _00198 Position (kb) 222 223 224Strain fitness (log2 ratio) -2 -1 0 1 2at 221.982 kb on - strand, within ECD_00194at 222.023 kb on + strand, within ECD_00194at 222.023 kb on + strand, within ECD_00194at 222.024 kb on - strand, within ECD_00194at 222.132 kb on + strand, within ECD_00194at 222.133 kb on - strand, within ECD_00194at 222.133 kb on - strand, within ECD_00194at 222.311 kb on + strand, within ECD_00194at 222.409 kb on + strandat 222.464 kb on + strandat 222.468 kb on + strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.515 kb on + strand, within ECD_00195at 222.569 kb on + strand, within ECD_00195at 222.681 kb on - strand, within ECD_00195at 222.683 kb on - strand, within ECD_00195at 222.810 kb on + strandat 222.811 kb on - strandat 222.837 kb on - strandat 222.837 kb on - strandat 222.852 kb on + strandat 222.853 kb on - strandat 222.929 kb on - strandat 222.929 kb on - strandat 223.022 kb on - strandat 223.118 kb on + strand, within ECD_00196at 223.156 kb on + strand, within ECD_00196at 223.156 kb on + strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.349 kb on + strand, within ECD_00196at 223.499 kb on - strand, within ECD_00196at 223.506 kb on - strand, within ECD_00196at 223.564 kb on + strand, within ECD_00196at 223.600 kb on + strand, within ECD_00196at 223.670 kb on + strand, within ECD_00196at 223.774 kb on + strandat 223.790 kb on - strandat 223.794 kb on - strandat 223.810 kb on + strandat 223.811 kb on - strandat 223.858 kb on - strandat 223.892 kb on + strand, within ECD_00197at 223.893 kb on - strand, within ECD_00197at 223.922 kb on - strand, within ECD_00197at 224.009 kb on + strand, within ECD_00197at 224.106 kb on + strand, within ECD_00197at 224.211 kb on - strand, within ECD_00197at 224.255 kb on + strand, within ECD_00197at 224.305 kb on + strand, within ECD_00197at 224.324 kb on + strand, within ECD_00197at 224.324 kb on + strand, within ECD_00197at 224.348 kb on - strand, within ECD_00197at 224.348 kb on - strand, within ECD_00197at 224.353 kb on - strand, within ECD_00197at 224.353 kb on - strand, within ECD_00197at 224.463 kb on - strandat 224.470 kb on + strandat 224.521 kb on - strandat 224.579 kb on + strand, within ECD_00198at 224.598 kb on + strand, within ECD_00198at 224.598 kb on + strand, within ECD_00198at 224.598 kb on + strand, within ECD_00198at 224.599 kb on - strand, within ECD_00198at 224.757 kb on + strand, within ECD_00198

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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221,982 - ECD_00194 0.36 -0.1
222,023 + ECD_00194 0.42 -0.2
222,023 + ECD_00194 0.42 -0.5
222,024 - ECD_00194 0.42 -0.8
222,132 + ECD_00194 0.57 -0.8
222,133 - ECD_00194 0.57 +0.3
222,133 - ECD_00194 0.57 +0.1
222,311 + ECD_00194 0.82 +0.4
222,409 + +0.2
222,464 + -0.8
222,468 + +0.6
222,469 - -0.6
222,469 - -0.3
222,469 - -0.4
222,469 - -0.1
222,515 + ECD_00195 0.20 -0.0
222,569 + ECD_00195 0.34 +0.1
222,681 - ECD_00195 0.61 +0.5
222,683 - ECD_00195 0.62 +0.7
222,810 + +0.8
222,811 - +0.1
222,837 - +0.3
222,837 - +0.0
222,852 + -0.1
222,853 - +0.4
222,929 - +0.3
222,929 - +0.1
223,022 - -2.0
223,118 + ECD_00196 0.20 -1.0
223,156 + ECD_00196 0.25 +1.0
223,156 + ECD_00196 0.25 -0.7
223,157 - ECD_00196 0.25 +0.5
223,157 - ECD_00196 0.25 -0.6
223,157 - ECD_00196 0.25 -1.1
223,157 - ECD_00196 0.25 +1.1
223,349 + ECD_00196 0.48 +0.3
223,499 - ECD_00196 0.67 -1.2
223,506 - ECD_00196 0.68 -1.5
223,564 + ECD_00196 0.75 +0.5
223,600 + ECD_00196 0.79 -1.4
223,670 + ECD_00196 0.88 +0.0
223,774 + -0.8
223,790 - -0.8
223,794 - -1.0
223,810 + -0.0
223,811 - +0.5
223,858 - +1.8
223,892 + ECD_00197 0.13 -0.2
223,893 - ECD_00197 0.13 +0.0
223,922 - ECD_00197 0.17 +0.1
224,009 + ECD_00197 0.31 -0.1
224,106 + ECD_00197 0.45 +0.4
224,211 - ECD_00197 0.61 +0.4
224,255 + ECD_00197 0.68 -1.2
224,305 + ECD_00197 0.76 -0.2
224,324 + ECD_00197 0.79 +0.2
224,324 + ECD_00197 0.79 +0.8
224,348 - ECD_00197 0.82 -0.7
224,348 - ECD_00197 0.82 +1.1
224,353 - ECD_00197 0.83 -0.3
224,353 - ECD_00197 0.83 +0.3
224,463 - -0.0
224,470 + -0.2
224,521 - -0.8
224,579 + ECD_00198 0.12 -0.2
224,598 + ECD_00198 0.14 -0.6
224,598 + ECD_00198 0.14 +0.2
224,598 + ECD_00198 0.14 +0.5
224,599 - ECD_00198 0.14 -0.0
224,757 + ECD_00198 0.29 -1.0

Or see this region's nucleotide sequence