Strain Fitness in Escherichia coli BL21 around ECD_r00003

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_r00002 and ECD_r00003 are separated by 188 nucleotidesECD_r00003 and ECD_t00003 are separated by 52 nucleotidesECD_t00003 and ECD_00200 are separated by 162 nucleotides ECD_r00002: ECD_r00002 - 23S ribosomal RNA, at 228,589 to 231,494 _r00002 ECD_r00003: ECD_r00003 - 5S ribosomal RNA, at 231,683 to 231,802 _r00003 ECD_t00003: ECD_t00003 - tRNA-Asp, at 231,855 to 231,931 _t00003 ECD_00200: ECD_00200 - 2,5-diketo-D-gluconate reductase B, at 232,094 to 232,897 _00200 Position (kb) 231 232Strain fitness (log2 ratio) -1 0 1at 231.934 kb on + strandat 231.934 kb on + strandat 231.937 kb on + strandat 232.362 kb on - strand, within ECD_00200at 232.424 kb on - strand, within ECD_00200at 232.566 kb on - strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.646 kb on - strand, within ECD_00200

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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231,934 + +0.1
231,934 + -0.2
231,937 + -0.4
232,362 - ECD_00200 0.33 -1.4
232,424 - ECD_00200 0.41 +1.6
232,566 - ECD_00200 0.59 +1.5
232,625 + ECD_00200 0.66 +0.1
232,625 + ECD_00200 0.66 +0.2
232,625 + ECD_00200 0.66 -0.4
232,626 - ECD_00200 0.66 +0.7
232,626 - ECD_00200 0.66 -0.3
232,646 - ECD_00200 0.69 +1.0

Or see this region's nucleotide sequence