Strain Fitness in Escherichia coli BL21 around ECD_01403

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01401 and ECD_04318 overlap by 4 nucleotidesECD_04318 and ECD_01402 are separated by 273 nucleotidesECD_01402 and ECD_01403 are separated by 85 nucleotidesECD_01403 and ECD_01404 are separated by 0 nucleotidesECD_01404 and ECD_01405 overlap by 4 nucleotides ECD_01401: ECD_01401 - gamma-aminobutyraldehyde dehydrogenase, at 1,469,641 to 1,471,065 _01401 ECD_04318: ECD_04318 - stress-induced small inner membrane enterobacterial protein, at 1,471,062 to 1,471,178 _04318 ECD_01402: ECD_01402 - DUF2566 family protein, at 1,471,452 to 1,471,625 _01402 ECD_01403: ECD_01403 - DUF2526 family protein, at 1,471,711 to 1,471,944 _01403 ECD_01404: ECD_01404 - DUF606 family inner membrane protein, at 1,471,945 to 1,472,394 _01404 ECD_01405: ECD_01405 - methionine N-acyltransferase; L-amino acid N-acyltransferase, at 1,472,391 to 1,472,909 _01405 Position (kb) 1471 1472Strain fitness (log2 ratio) -1 0 1 2at 1470.927 kb on + strandat 1471.069 kb on + strandat 1471.292 kb on - strandat 1471.630 kb on + strandat 1471.710 kb on + strandat 1471.787 kb on + strand, within ECD_01403at 1471.787 kb on + strand, within ECD_01403at 1471.817 kb on + strand, within ECD_01403at 1472.173 kb on - strand, within ECD_01404at 1472.173 kb on - strand, within ECD_01404at 1472.173 kb on - strand, within ECD_01404at 1472.746 kb on - strand, within ECD_01405at 1472.840 kb on - strand, within ECD_01405at 1472.840 kb on - strand, within ECD_01405

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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1,470,927 + -0.2
1,471,069 + -0.3
1,471,292 - +1.5
1,471,630 + -0.1
1,471,710 + -0.2
1,471,787 + ECD_01403 0.32 -1.1
1,471,787 + ECD_01403 0.32 +1.1
1,471,817 + ECD_01403 0.45 +0.1
1,472,173 - ECD_01404 0.51 -0.5
1,472,173 - ECD_01404 0.51 +0.4
1,472,173 - ECD_01404 0.51 +1.8
1,472,746 - ECD_01405 0.68 +1.2
1,472,840 - ECD_01405 0.87 -0.6
1,472,840 - ECD_01405 0.87 +0.9

Or see this region's nucleotide sequence