Strain Fitness in Escherichia coli BL21 around ECD_00154

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00153 and ECD_00154 are separated by 224 nucleotidesECD_00154 and ECD_00155 are separated by 81 nucleotidesECD_00155 and ECD_00156 are separated by 46 nucleotides ECD_00153: ECD_00153 - glutamate-1-semialdehyde aminotransferase (aminomutase), at 176,445 to 177,725 _00153 ECD_00154: ECD_00154 - H(+)/Cl(-) exchange transporter, at 177,950 to 179,371 _00154 ECD_00155: ECD_00155 - iron-sulfur cluster insertion protein, at 179,453 to 179,797 _00155 ECD_00156: ECD_00156 - UPF0126 family inner membrane protein, at 179,844 to 180,467 _00156 Position (kb) 177 178 179 180Strain fitness (log2 ratio) -2 -1 0 1at 177.768 kb on + strandat 177.768 kb on + strandat 177.768 kb on + strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.769 kb on - strandat 177.779 kb on + strandat 177.846 kb on - strandat 177.870 kb on + strandat 177.927 kb on + strandat 177.972 kb on + strandat 178.061 kb on - strandat 178.141 kb on + strand, within ECD_00154at 178.156 kb on - strand, within ECD_00154at 178.156 kb on - strand, within ECD_00154at 178.159 kb on + strand, within ECD_00154at 178.160 kb on - strand, within ECD_00154at 178.169 kb on - strand, within ECD_00154at 178.429 kb on + strand, within ECD_00154at 178.429 kb on + strand, within ECD_00154at 178.475 kb on - strand, within ECD_00154at 178.578 kb on - strand, within ECD_00154at 178.595 kb on - strand, within ECD_00154at 178.607 kb on - strand, within ECD_00154at 178.618 kb on + strand, within ECD_00154at 178.618 kb on + strand, within ECD_00154at 178.630 kb on + strand, within ECD_00154at 178.630 kb on + strand, within ECD_00154at 178.631 kb on - strand, within ECD_00154at 178.631 kb on - strand, within ECD_00154at 178.631 kb on - strand, within ECD_00154at 178.631 kb on - strand, within ECD_00154at 178.697 kb on - strand, within ECD_00154at 178.713 kb on - strand, within ECD_00154at 178.827 kb on + strand, within ECD_00154at 178.983 kb on + strand, within ECD_00154at 178.983 kb on + strand, within ECD_00154at 178.983 kb on + strand, within ECD_00154at 178.984 kb on - strand, within ECD_00154at 179.120 kb on - strand, within ECD_00154at 179.152 kb on + strand, within ECD_00154at 179.153 kb on - strand, within ECD_00154at 179.204 kb on + strand, within ECD_00154at 179.205 kb on - strand, within ECD_00154at 179.227 kb on + strand, within ECD_00154at 179.228 kb on - strand, within ECD_00154at 179.250 kb on + strandat 179.251 kb on - strandat 179.251 kb on - strandat 179.390 kb on - strandat 179.393 kb on + strandat 179.452 kb on + strandat 179.788 kb on + strandat 179.789 kb on - strandat 179.791 kb on + strandat 179.791 kb on + strandat 179.882 kb on + strandat 179.882 kb on + strandat 179.883 kb on - strandat 179.883 kb on - strandat 179.974 kb on + strand, within ECD_00156at 180.006 kb on - strand, within ECD_00156at 180.092 kb on + strand, within ECD_00156at 180.104 kb on + strand, within ECD_00156at 180.137 kb on + strand, within ECD_00156at 180.301 kb on - strand, within ECD_00156at 180.345 kb on + strand, within ECD_00156

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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177,768 + -0.7
177,768 + -0.5
177,768 + -0.0
177,769 - +0.1
177,769 - +0.7
177,769 - +0.9
177,769 - +0.6
177,769 - -0.8
177,769 - +0.3
177,769 - -0.3
177,779 + +0.3
177,846 - -0.3
177,870 + +1.1
177,927 + -0.6
177,972 + +0.2
178,061 - +0.2
178,141 + ECD_00154 0.13 -0.4
178,156 - ECD_00154 0.14 +0.8
178,156 - ECD_00154 0.14 -0.2
178,159 + ECD_00154 0.15 -0.2
178,160 - ECD_00154 0.15 +0.4
178,169 - ECD_00154 0.15 +0.1
178,429 + ECD_00154 0.34 -0.2
178,429 + ECD_00154 0.34 +0.0
178,475 - ECD_00154 0.37 +0.9
178,578 - ECD_00154 0.44 -0.5
178,595 - ECD_00154 0.45 +0.8
178,607 - ECD_00154 0.46 -0.1
178,618 + ECD_00154 0.47 -0.4
178,618 + ECD_00154 0.47 -0.3
178,630 + ECD_00154 0.48 -1.5
178,630 + ECD_00154 0.48 +1.5
178,631 - ECD_00154 0.48 +0.8
178,631 - ECD_00154 0.48 -0.0
178,631 - ECD_00154 0.48 +0.0
178,631 - ECD_00154 0.48 +0.3
178,697 - ECD_00154 0.53 +0.1
178,713 - ECD_00154 0.54 -0.2
178,827 + ECD_00154 0.62 +0.2
178,983 + ECD_00154 0.73 -0.8
178,983 + ECD_00154 0.73 -0.2
178,983 + ECD_00154 0.73 -0.1
178,984 - ECD_00154 0.73 +0.3
179,120 - ECD_00154 0.82 -2.1
179,152 + ECD_00154 0.85 +0.1
179,153 - ECD_00154 0.85 -0.6
179,204 + ECD_00154 0.88 -0.7
179,205 - ECD_00154 0.88 +0.2
179,227 + ECD_00154 0.90 +0.2
179,228 - ECD_00154 0.90 +0.6
179,250 + -0.7
179,251 - +0.1
179,251 - +0.2
179,390 - -0.5
179,393 + +0.5
179,452 + +0.6
179,788 + -0.3
179,789 - -0.0
179,791 + +0.7
179,791 + -0.1
179,882 + +1.7
179,882 + -0.6
179,883 - +0.9
179,883 - -0.1
179,974 + ECD_00156 0.21 +0.8
180,006 - ECD_00156 0.26 -1.4
180,092 + ECD_00156 0.40 -0.9
180,104 + ECD_00156 0.42 -1.1
180,137 + ECD_00156 0.47 +0.1
180,301 - ECD_00156 0.73 -0.5
180,345 + ECD_00156 0.80 +0.3

Or see this region's nucleotide sequence