Strain Fitness in Escherichia coli BL21 around ECD_00116

Experiment: N4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00115 and ECD_00116 are separated by 70 nucleotidesECD_00116 and ECD_00117 are separated by 354 nucleotides ECD_00115: ECD_00115 - dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase, at 130,717 to 132,141 _00115 ECD_00116: ECD_00116 - DUF3300 family protein, at 132,212 to 134,065 _00116 ECD_00117: ECD_00117 - aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase, at 134,420 to 137,017 _00117 Position (kb) 132 133 134 135Strain fitness (log2 ratio) -1 0 1 2at 132.140 kb on - strandat 132.170 kb on + strandat 132.170 kb on + strandat 132.293 kb on - strandat 132.298 kb on + strandat 132.739 kb on - strand, within ECD_00116at 132.748 kb on - strand, within ECD_00116at 132.763 kb on + strand, within ECD_00116at 132.951 kb on + strand, within ECD_00116at 132.970 kb on - strand, within ECD_00116at 132.970 kb on - strand, within ECD_00116at 132.995 kb on - strand, within ECD_00116at 133.006 kb on + strand, within ECD_00116at 133.006 kb on + strand, within ECD_00116at 133.113 kb on + strand, within ECD_00116at 133.113 kb on + strand, within ECD_00116at 133.114 kb on - strand, within ECD_00116at 133.175 kb on + strand, within ECD_00116at 133.175 kb on + strand, within ECD_00116at 133.183 kb on - strand, within ECD_00116at 133.204 kb on + strand, within ECD_00116at 133.205 kb on - strand, within ECD_00116at 133.205 kb on - strand, within ECD_00116at 133.264 kb on - strand, within ECD_00116at 133.318 kb on - strand, within ECD_00116at 133.329 kb on + strand, within ECD_00116at 133.329 kb on + strand, within ECD_00116at 133.412 kb on - strand, within ECD_00116at 133.474 kb on + strand, within ECD_00116at 133.541 kb on + strand, within ECD_00116at 133.542 kb on - strand, within ECD_00116at 133.721 kb on - strand, within ECD_00116at 133.791 kb on - strand, within ECD_00116at 133.833 kb on - strand, within ECD_00116at 133.907 kb on - strandat 133.980 kb on - strandat 133.980 kb on - strandat 133.981 kb on + strandat 133.981 kb on + strandat 133.986 kb on - strandat 134.133 kb on + strandat 134.134 kb on - strandat 134.134 kb on - strandat 134.134 kb on - strandat 134.235 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4
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132,140 - -0.4
132,170 + -0.5
132,170 + -0.5
132,293 - -1.2
132,298 + +0.5
132,739 - ECD_00116 0.28 +0.9
132,748 - ECD_00116 0.29 -0.7
132,763 + ECD_00116 0.30 +0.0
132,951 + ECD_00116 0.40 +1.0
132,970 - ECD_00116 0.41 -0.2
132,970 - ECD_00116 0.41 +0.1
132,995 - ECD_00116 0.42 +0.1
133,006 + ECD_00116 0.43 -0.7
133,006 + ECD_00116 0.43 +0.6
133,113 + ECD_00116 0.49 +0.3
133,113 + ECD_00116 0.49 +0.2
133,114 - ECD_00116 0.49 +0.1
133,175 + ECD_00116 0.52 -0.9
133,175 + ECD_00116 0.52 +0.8
133,183 - ECD_00116 0.52 +0.1
133,204 + ECD_00116 0.54 +0.6
133,205 - ECD_00116 0.54 +0.4
133,205 - ECD_00116 0.54 +0.4
133,264 - ECD_00116 0.57 -0.2
133,318 - ECD_00116 0.60 -0.7
133,329 + ECD_00116 0.60 +0.4
133,329 + ECD_00116 0.60 -1.2
133,412 - ECD_00116 0.65 +0.0
133,474 + ECD_00116 0.68 +1.3
133,541 + ECD_00116 0.72 +0.3
133,542 - ECD_00116 0.72 -0.7
133,721 - ECD_00116 0.81 -0.0
133,791 - ECD_00116 0.85 +0.8
133,833 - ECD_00116 0.87 +0.9
133,907 - -0.4
133,980 - +1.0
133,980 - +0.2
133,981 + +1.5
133,981 + +0.0
133,986 - +0.2
134,133 + +0.5
134,134 - +0.8
134,134 - +0.1
134,134 - -1.6
134,235 + +1.8

Or see this region's nucleotide sequence