Strain Fitness in Escherichia coli BL21 around ECD_01841

Experiment: Laval1261

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01839 and ECD_01840 are separated by 52 nucleotidesECD_01840 and ECD_01841 are separated by 40 nucleotidesECD_01841 and ECD_01842 overlap by 4 nucleotides ECD_01839: ECD_01839 - UPF0759 family protein, at 1,897,313 to 1,898,131 _01839 ECD_01840: ECD_01840 - MAPEG family inner membrane protein, at 1,898,184 to 1,898,579 _01840 ECD_01841: ECD_01841 - carboxy-SAM synthase, at 1,898,620 to 1,899,363 _01841 ECD_01842: ECD_01842 - tRNA (cmo5U34)-carboxymethyltransferase, carboxy-SAM-dependent, at 1,899,360 to 1,900,331 _01842 Position (kb) 1898 1899 1900Strain fitness (log2 ratio) -3 -2 -1 0 1at 1897.927 kb on + strand, within ECD_01839at 1898.050 kb on + strandat 1898.142 kb on + strandat 1898.147 kb on + strandat 1898.147 kb on + strandat 1898.199 kb on + strandat 1898.199 kb on + strandat 1898.209 kb on + strandat 1898.546 kb on + strandat 1898.587 kb on + strandat 1898.587 kb on + strandat 1898.734 kb on + strand, within ECD_01841at 1898.760 kb on + strand, within ECD_01841at 1898.761 kb on - strand, within ECD_01841at 1898.922 kb on + strand, within ECD_01841at 1898.951 kb on + strand, within ECD_01841at 1899.444 kb on - strandat 1899.522 kb on + strand, within ECD_01842at 1899.587 kb on + strand, within ECD_01842at 1899.648 kb on + strand, within ECD_01842

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1261
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1,897,927 + ECD_01839 0.75 +0.8
1,898,050 + +0.2
1,898,142 + +0.1
1,898,147 + +0.2
1,898,147 + +0.1
1,898,199 + +0.2
1,898,199 + +0.5
1,898,209 + -0.0
1,898,546 + +0.4
1,898,587 + +1.2
1,898,587 + -2.9
1,898,734 + ECD_01841 0.15 -0.7
1,898,760 + ECD_01841 0.19 -0.4
1,898,761 - ECD_01841 0.19 +0.7
1,898,922 + ECD_01841 0.41 +1.2
1,898,951 + ECD_01841 0.44 +0.7
1,899,444 - +0.2
1,899,522 + ECD_01842 0.17 -0.6
1,899,587 + ECD_01842 0.23 +0.7
1,899,648 + ECD_01842 0.30 -2.4

Or see this region's nucleotide sequence