Strain Fitness in Escherichia coli BL21 around ECD_01615

Experiment: Laval1261

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01613 and ECD_01614 are separated by 2 nucleotidesECD_01614 and ECD_01615 overlap by 1 nucleotidesECD_01615 and ECD_01616 are separated by 0 nucleotidesECD_01616 and ECD_01617 are separated by 80 nucleotides ECD_01613: ECD_01613 - DUF1656 family putative inner membrane efflux pump associated protein, at 1,666,221 to 1,666,457 _01613 ECD_01614: ECD_01614 - putative membrane fusion protein (MFP) of YdhJK efflux pump, at 1,666,460 to 1,667,317 _01614 ECD_01615: ECD_01615 - putative efflux protein (PET) component of YdhJK efflux pump, at 1,667,317 to 1,669,329 _01615 ECD_01616: ECD_01616 - superoxide dismutase, Cu, Zn, periplasmic, at 1,669,330 to 1,669,851 _01616 ECD_01617: ECD_01617 - putative oxidoreductase, at 1,669,932 to 1,670,828 _01617 Position (kb) 1667 1668 1669 1670Strain fitness (log2 ratio) -1 0 1 2at 1666.340 kb on - strand, within ECD_01613at 1666.621 kb on + strand, within ECD_01614at 1666.622 kb on - strand, within ECD_01614at 1666.636 kb on + strand, within ECD_01614at 1667.096 kb on + strand, within ECD_01614at 1667.885 kb on + strand, within ECD_01615at 1668.062 kb on + strand, within ECD_01615at 1668.063 kb on - strand, within ECD_01615at 1668.309 kb on - strand, within ECD_01615at 1668.329 kb on + strand, within ECD_01615at 1668.965 kb on - strand, within ECD_01615at 1669.396 kb on - strand, within ECD_01616at 1669.840 kb on - strandat 1670.165 kb on - strand, within ECD_01617

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1261
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1,666,340 - ECD_01613 0.50 +0.1
1,666,621 + ECD_01614 0.19 +0.5
1,666,622 - ECD_01614 0.19 -0.2
1,666,636 + ECD_01614 0.21 +0.4
1,667,096 + ECD_01614 0.74 +0.4
1,667,885 + ECD_01615 0.28 +0.6
1,668,062 + ECD_01615 0.37 +1.8
1,668,063 - ECD_01615 0.37 +0.9
1,668,309 - ECD_01615 0.49 +0.2
1,668,329 + ECD_01615 0.50 +0.9
1,668,965 - ECD_01615 0.82 +0.7
1,669,396 - ECD_01616 0.13 -0.6
1,669,840 - +0.7
1,670,165 - ECD_01617 0.26 +0.8

Or see this region's nucleotide sequence