Strain Fitness in Escherichia coli BL21 around ECD_02196

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02195 and ECD_02196 are separated by 187 nucleotides ECD_02195: ECD_02195 - RNase BN, tRNA processing enzyme, at 2,271,782 to 2,272,699 _02195 ECD_02196: ECD_02196 - protease, capable of cleaving an AMC-ubiquitin model substrate, at 2,272,887 to 2,274,098 _02196 Position (kb) 2272 2273 2274 2275Strain fitness (log2 ratio) -1 0 1at 2272.025 kb on - strand, within ECD_02195at 2272.218 kb on - strand, within ECD_02195at 2272.229 kb on - strand, within ECD_02195at 2272.258 kb on - strand, within ECD_02195at 2272.273 kb on - strand, within ECD_02195at 2272.375 kb on - strand, within ECD_02195at 2272.375 kb on - strand, within ECD_02195at 2272.491 kb on + strand, within ECD_02195at 2272.759 kb on - strandat 2272.759 kb on - strandat 2272.799 kb on + strandat 2273.040 kb on - strand, within ECD_02196at 2274.683 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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2,272,025 - ECD_02195 0.26 +0.2
2,272,218 - ECD_02195 0.47 +0.9
2,272,229 - ECD_02195 0.49 +0.0
2,272,258 - ECD_02195 0.52 -0.2
2,272,273 - ECD_02195 0.53 +1.3
2,272,375 - ECD_02195 0.65 -0.0
2,272,375 - ECD_02195 0.65 +1.2
2,272,491 + ECD_02195 0.77 -1.3
2,272,759 - -0.4
2,272,759 - -0.3
2,272,799 + -0.2
2,273,040 - ECD_02196 0.13 -0.2
2,274,683 + +0.2

Or see this region's nucleotide sequence