Experiment: EV152
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01009 and ECD_01010 are separated by 20 nucleotides ECD_01010 and ECD_01011 are separated by 10 nucleotides ECD_01011 and ECD_01012 are separated by 9 nucleotides ECD_01012 and ECD_01013 are separated by 7 nucleotides
ECD_01009: ECD_01009 - pyrimidine permease, at 1,072,793 to 1,074,121
_01009
ECD_01010: ECD_01010 - flavin:NADH reductase, at 1,074,142 to 1,074,636
_01010
ECD_01011: ECD_01011 - putative malonic semialdehyde reductase, at 1,074,647 to 1,075,237
_01011
ECD_01012: ECD_01012 - putative reactive intermediate detoxifying aminoacrylate hydrolase, at 1,075,247 to 1,076,047
_01012
ECD_01013: ECD_01013 - putative aminoacrylate deaminase, reactive intermediate detoxification; weak enamine/imine deaminase activity, at 1,076,055 to 1,076,441
_01013
Position (kb) 1074
1075
1076 Strain fitness (log2 ratio) -1
0
1
2 at 1073.755 kb on - strand, within ECD_01009 at 1073.933 kb on + strand, within ECD_01009 at 1073.975 kb on + strand, within ECD_01009 at 1074.143 kb on + strand at 1074.649 kb on - strand at 1074.727 kb on + strand, within ECD_01011 at 1075.129 kb on - strand, within ECD_01011 at 1075.271 kb on + strand at 1075.272 kb on - strand at 1075.477 kb on + strand, within ECD_01012 at 1075.501 kb on + strand, within ECD_01012 at 1075.867 kb on - strand, within ECD_01012 at 1075.892 kb on - strand, within ECD_01012 at 1076.204 kb on + strand, within ECD_01013
Per-strain Table
Position Strand Gene LocusTag Fraction EV152 remove 1,073,755 - ECD_01009 0.72 +0.8 1,073,933 + ECD_01009 0.86 -0.9 1,073,975 + ECD_01009 0.89 -0.0 1,074,143 + -0.7 1,074,649 - -0.5 1,074,727 + ECD_01011 0.14 -0.3 1,075,129 - ECD_01011 0.82 -1.0 1,075,271 + -0.3 1,075,272 - +0.6 1,075,477 + ECD_01012 0.29 +2.2 1,075,501 + ECD_01012 0.32 +0.2 1,075,867 - ECD_01012 0.77 +2.6 1,075,892 - ECD_01012 0.81 -0.4 1,076,204 + ECD_01013 0.39 -0.4
Or see this region's nucleotide sequence