Strain Fitness in Escherichia coli BL21 around ECD_00923

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00921 and ECD_00922 overlap by 4 nucleotidesECD_00922 and ECD_00923 are separated by 153 nucleotidesECD_00923 and ECD_00924 overlap by 24 nucleotidesECD_00924 and ECD_00925 are separated by 135 nucleotides ECD_00921: ECD_00921 - peroxide and acid resistance protein, UPF0434 family, at 975,763 to 975,945 _00921 ECD_00922: ECD_00922 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 975,942 to 976,688 _00922 ECD_00923: ECD_00923 - protein kinase-like domain protein, at 976,842 to 977,735 _00923 ECD_00924: ECD_00924 - envelope biogenesis factor; DUF218 superfamily protein, at 977,712 to 978,491 _00924 ECD_00925: ECD_00925 - putative S-adenosyl-L-methionine-dependent methyltransferase, at 978,627 to 979,412 _00925 Position (kb) 976 977 978Strain fitness (log2 ratio) -2 -1 0 1 2at 976.942 kb on - strand, within ECD_00923at 977.068 kb on - strand, within ECD_00923at 977.068 kb on - strand, within ECD_00923at 977.204 kb on - strand, within ECD_00923at 977.396 kb on - strand, within ECD_00923at 977.634 kb on + strand, within ECD_00923at 978.183 kb on - strand, within ECD_00924at 978.516 kb on + strandat 978.516 kb on + strandat 978.518 kb on + strandat 978.518 kb on + strandat 978.518 kb on + strandat 978.519 kb on - strandat 978.538 kb on + strandat 978.538 kb on + strandat 978.541 kb on + strandat 978.541 kb on + strandat 978.541 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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976,942 - ECD_00923 0.11 -1.4
977,068 - ECD_00923 0.25 -1.0
977,068 - ECD_00923 0.25 +0.8
977,204 - ECD_00923 0.40 +0.6
977,396 - ECD_00923 0.62 -1.0
977,634 + ECD_00923 0.89 -1.1
978,183 - ECD_00924 0.60 +1.6
978,516 + -2.2
978,516 + -1.1
978,518 + -1.1
978,518 + -1.2
978,518 + -0.9
978,519 - -0.6
978,538 + -0.9
978,538 + -0.3
978,541 + +0.3
978,541 + +2.5
978,541 + -0.5

Or see this region's nucleotide sequence