Strain Fitness in Escherichia coli BL21 around ECD_00830

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00828 and ECD_00829 are separated by 271 nucleotidesECD_00829 and ECD_00830 are separated by 36 nucleotidesECD_00830 and ECD_00831 are separated by 162 nucleotides ECD_00828: ECD_00828 - hypothetical protein, at 877,081 to 877,416 _00828 ECD_00829: ECD_00829 - hypothetical protein, at 877,688 to 877,852 _00829 ECD_00830: ECD_00830 - hypothetical protein, at 877,889 to 878,812 _00830 ECD_00831: ECD_00831 - retron EC86 RNA-directed DNA polymerase-like protein, at 878,975 to 879,937 _00831 Position (kb) 877 878 879Strain fitness (log2 ratio) -2 -1 0 1at 876.948 kb on + strandat 877.077 kb on - strandat 877.083 kb on - strandat 877.184 kb on - strand, within ECD_00828at 877.351 kb on - strand, within ECD_00828at 877.501 kb on - strandat 877.555 kb on + strandat 877.760 kb on - strand, within ECD_00829at 878.693 kb on + strand, within ECD_00830at 878.698 kb on + strand, within ECD_00830at 878.779 kb on + strandat 878.779 kb on + strandat 878.828 kb on + strandat 878.877 kb on + strandat 878.877 kb on + strandat 878.957 kb on + strandat 879.125 kb on + strand, within ECD_00831at 879.198 kb on + strand, within ECD_00831at 879.655 kb on + strand, within ECD_00831

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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876,948 + +1.3
877,077 - +0.6
877,083 - -0.0
877,184 - ECD_00828 0.31 -0.8
877,351 - ECD_00828 0.80 +0.5
877,501 - +1.1
877,555 + +0.3
877,760 - ECD_00829 0.44 +0.8
878,693 + ECD_00830 0.87 -0.6
878,698 + ECD_00830 0.88 -0.9
878,779 + -1.6
878,779 + -0.3
878,828 + -0.8
878,877 + -1.8
878,877 + -0.8
878,957 + +0.2
879,125 + ECD_00831 0.16 -1.2
879,198 + ECD_00831 0.23 -0.7
879,655 + ECD_00831 0.71 -1.2

Or see this region's nucleotide sequence