Strain Fitness in Escherichia coli BL21 around ECD_00539

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00538 and ECD_00539 are separated by 107 nucleotidesECD_00539 and ECD_00540 are separated by 93 nucleotidesECD_00540 and ECD_00541 are separated by 64 nucleotidesECD_00541 and ECD_00542 are separated by 65 nucleotides ECD_00538: ECD_00538 - mechanosensitive channel protein, miniconductance, at 562,085 to 563,332 _00538 ECD_00539: ECD_00539 - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, at 563,440 to 564,093 _00539 ECD_00540: ECD_00540 - DUF419 family protein, at 564,187 to 564,555 _00540 ECD_00541: ECD_00541 - DUF1158 family putative inner membrane protein, at 564,620 to 564,868 _00541 ECD_00542: ECD_00542 - weak gamma-glutamyl:cysteine ligase, at 564,934 to 566,052 _00542 Position (kb) 563 564 565Strain fitness (log2 ratio) -1 0 1at 562.449 kb on - strand, within ECD_00538at 562.733 kb on - strand, within ECD_00538at 562.754 kb on - strand, within ECD_00538at 562.754 kb on - strand, within ECD_00538at 562.783 kb on + strand, within ECD_00538at 562.784 kb on - strand, within ECD_00538at 562.914 kb on - strand, within ECD_00538at 562.970 kb on - strand, within ECD_00538at 563.322 kb on - strandat 563.322 kb on - strandat 563.332 kb on + strandat 563.346 kb on + strandat 563.347 kb on - strandat 563.547 kb on - strand, within ECD_00539at 563.716 kb on - strand, within ECD_00539at 563.874 kb on + strand, within ECD_00539at 563.874 kb on + strand, within ECD_00539at 563.891 kb on + strand, within ECD_00539at 563.892 kb on - strand, within ECD_00539at 563.949 kb on + strand, within ECD_00539at 563.988 kb on - strand, within ECD_00539at 564.156 kb on - strandat 564.176 kb on - strandat 564.176 kb on - strandat 564.356 kb on - strand, within ECD_00540at 564.436 kb on - strand, within ECD_00540at 564.590 kb on + strandat 564.590 kb on + strandat 564.591 kb on - strandat 564.670 kb on - strand, within ECD_00541at 564.868 kb on + strandat 564.884 kb on - strandat 564.935 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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562,449 - ECD_00538 0.29 -0.4
562,733 - ECD_00538 0.52 -0.5
562,754 - ECD_00538 0.54 +0.5
562,754 - ECD_00538 0.54 +1.3
562,783 + ECD_00538 0.56 -1.5
562,784 - ECD_00538 0.56 +0.3
562,914 - ECD_00538 0.66 -0.3
562,970 - ECD_00538 0.71 -0.2
563,322 - +1.1
563,322 - -0.2
563,332 + -1.1
563,346 + +0.3
563,347 - +0.1
563,547 - ECD_00539 0.16 +0.2
563,716 - ECD_00539 0.42 +1.0
563,874 + ECD_00539 0.66 +1.6
563,874 + ECD_00539 0.66 +0.6
563,891 + ECD_00539 0.69 +0.3
563,892 - ECD_00539 0.69 +1.0
563,949 + ECD_00539 0.78 +1.1
563,988 - ECD_00539 0.84 -1.7
564,156 - -0.3
564,176 - +0.1
564,176 - -0.4
564,356 - ECD_00540 0.46 +0.8
564,436 - ECD_00540 0.67 +0.2
564,590 + -0.3
564,590 + +0.5
564,591 - +0.4
564,670 - ECD_00541 0.20 -0.5
564,868 + -0.4
564,884 - +0.2
564,935 + -0.8

Or see this region's nucleotide sequence