Strain Fitness in Escherichia coli BL21 around ECD_00112

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00111 and ECD_00112 are separated by 540 nucleotidesECD_00112 and ECD_00113 are separated by 160 nucleotides ECD_00111: ECD_00111 - aromatic amino acid transporter, at 122,983 to 124,356 _00111 ECD_00112: ECD_00112 - pyruvate dehydrogenase complex repressor; autorepressor, at 124,897 to 125,661 _00112 ECD_00113: ECD_00113 - pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate-binding, at 125,822 to 128,485 _00113 Position (kb) 124 125 126Strain fitness (log2 ratio) -2 -1 0 1at 124.007 kb on + strand, within ECD_00111at 124.008 kb on - strand, within ECD_00111at 124.012 kb on - strand, within ECD_00111at 124.235 kb on - strandat 124.235 kb on - strandat 124.235 kb on - strandat 124.291 kb on - strandat 124.299 kb on + strandat 124.389 kb on - strandat 124.708 kb on + strandat 124.728 kb on - strandat 124.731 kb on + strandat 124.749 kb on + strandat 124.750 kb on - strandat 124.751 kb on + strandat 124.883 kb on + strandat 124.903 kb on + strandat 124.903 kb on + strandat 125.188 kb on + strand, within ECD_00112at 125.188 kb on + strand, within ECD_00112at 125.188 kb on + strand, within ECD_00112at 125.223 kb on + strand, within ECD_00112at 125.233 kb on + strand, within ECD_00112at 125.236 kb on + strand, within ECD_00112at 125.405 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.412 kb on + strand, within ECD_00112at 125.461 kb on + strand, within ECD_00112at 125.510 kb on + strand, within ECD_00112at 125.733 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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124,007 + ECD_00111 0.75 -0.0
124,008 - ECD_00111 0.75 -0.5
124,012 - ECD_00111 0.75 +0.2
124,235 - +0.8
124,235 - +0.5
124,235 - -1.1
124,291 - -0.6
124,299 + -0.7
124,389 - -0.4
124,708 + -0.2
124,728 - +0.0
124,731 + +0.2
124,749 + -0.8
124,750 - -1.4
124,751 + +0.0
124,883 + +0.9
124,903 + -0.7
124,903 + -1.1
125,188 + ECD_00112 0.38 +0.8
125,188 + ECD_00112 0.38 -1.0
125,188 + ECD_00112 0.38 +0.6
125,223 + ECD_00112 0.43 -0.9
125,233 + ECD_00112 0.44 -1.1
125,236 + ECD_00112 0.44 -1.3
125,405 + ECD_00112 0.66 -0.7
125,412 + ECD_00112 0.67 -0.7
125,412 + ECD_00112 0.67 -1.7
125,412 + ECD_00112 0.67 -0.9
125,412 + ECD_00112 0.67 -2.7
125,412 + ECD_00112 0.67 -1.4
125,461 + ECD_00112 0.74 -0.3
125,510 + ECD_00112 0.80 -0.1
125,733 + +0.0

Or see this region's nucleotide sequence