Strain Fitness in Escherichia coli BL21 around ECD_01385

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01382 and ECD_01383 are separated by 15 nucleotidesECD_01383 and ECD_01384 are separated by 62 nucleotidesECD_01384 and ECD_01385 overlap by 9 nucleotidesECD_01385 and ECD_01386 are separated by 123 nucleotides ECD_01381: ECD_01381 - OPG biosynthetic periplasmic protein, at 1,450,948 to 1,452,573 _01381 ECD_01382: ECD_01382 - hypothetical protein, at 1,452,494 to 1,452,697 _01382 ECD_01383: ECD_01383 - DUF465 family protein, at 1,452,713 to 1,452,937 _01383 ECD_01384: ECD_01384 - ribosomal-protein-L7/L12-serine acetyltransferase, at 1,453,000 to 1,453,539 _01384 ECD_01385: ECD_01385 - uncharacterized protein, at 1,453,531 to 1,454,511 _01385 ECD_01386: ECD_01386 - potassium-tellurite ethidium and proflavin transporter, at 1,454,635 to 1,455,627 _01386 Position (kb) 1453 1454 1455Strain fitness (log2 ratio) -1 0 1at 1452.547 kb on + strandat 1452.572 kb on - strandat 1454.396 kb on - strand, within ECD_01385at 1454.679 kb on + strandat 1455.377 kb on - strand, within ECD_01386

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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1,452,547 + -1.1
1,452,572 - -0.8
1,454,396 - ECD_01385 0.88 +1.5
1,454,679 + +0.1
1,455,377 - ECD_01386 0.75 -0.8

Or see this region's nucleotide sequence