Strain Fitness in Escherichia coli BL21 around ECD_00217

Experiment: EV152

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00216 and ECD_00217 are separated by 239 nucleotidesECD_00217 and ECD_00218 are separated by 205 nucleotidesECD_00218 and ECD_00219 overlap by 30 nucleotides ECD_00216: ECD_00216 - acyl coenzyme A dehydrogenase, at 245,433 to 247,877 _00216 ECD_00217: ECD_00217 - D-sedoheptulose 7-phosphate isomerase, at 248,117 to 248,695 _00217 ECD_00218: ECD_00218 - type 2 glutamine amidotransferase family protein, at 248,901 to 249,668 _00218 ECD_00219: ECD_00219 - L,D-transpeptidase-related protein, at 249,639 to 250,379 _00219 Position (kb) 248 249Strain fitness (log2 ratio) -2 -1 0 1at 247.257 kb on + strand, within ECD_00216at 247.334 kb on + strand, within ECD_00216at 247.337 kb on - strand, within ECD_00216at 247.361 kb on - strand, within ECD_00216at 247.406 kb on - strand, within ECD_00216at 247.534 kb on + strand, within ECD_00216at 247.538 kb on + strand, within ECD_00216at 247.538 kb on + strand, within ECD_00216at 247.539 kb on - strand, within ECD_00216at 247.539 kb on - strand, within ECD_00216at 247.663 kb on - strandat 247.686 kb on - strandat 247.810 kb on - strandat 247.951 kb on - strandat 247.974 kb on - strandat 248.092 kb on + strandat 248.121 kb on - strandat 248.377 kb on + strand, within ECD_00217at 248.776 kb on - strandat 248.968 kb on + strandat 248.968 kb on + strandat 248.968 kb on + strandat 248.996 kb on + strand, within ECD_00218at 248.997 kb on - strand, within ECD_00218at 249.021 kb on + strand, within ECD_00218at 249.048 kb on - strand, within ECD_00218at 249.068 kb on + strand, within ECD_00218at 249.213 kb on + strand, within ECD_00218at 249.213 kb on + strand, within ECD_00218at 249.374 kb on + strand, within ECD_00218at 249.442 kb on - strand, within ECD_00218at 249.442 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.465 kb on - strand, within ECD_00218at 249.661 kb on + strandat 249.662 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV152
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247,257 + ECD_00216 0.75 +0.8
247,334 + ECD_00216 0.78 -0.3
247,337 - ECD_00216 0.78 +0.6
247,361 - ECD_00216 0.79 +0.6
247,406 - ECD_00216 0.81 +0.9
247,534 + ECD_00216 0.86 +0.3
247,538 + ECD_00216 0.86 +1.4
247,538 + ECD_00216 0.86 -0.3
247,539 - ECD_00216 0.86 -0.3
247,539 - ECD_00216 0.86 +0.1
247,663 - +0.9
247,686 - -0.8
247,810 - -0.5
247,951 - +0.4
247,974 - +1.7
248,092 + +1.0
248,121 - +1.0
248,377 + ECD_00217 0.45 +1.0
248,776 - +1.0
248,968 + -0.7
248,968 + -0.2
248,968 + +0.8
248,996 + ECD_00218 0.12 -1.0
248,997 - ECD_00218 0.12 +0.4
249,021 + ECD_00218 0.16 +0.2
249,048 - ECD_00218 0.19 +0.4
249,068 + ECD_00218 0.22 -1.8
249,213 + ECD_00218 0.41 +0.6
249,213 + ECD_00218 0.41 -1.3
249,374 + ECD_00218 0.62 -0.2
249,442 - ECD_00218 0.70 +1.0
249,442 - ECD_00218 0.70 +0.3
249,446 - ECD_00218 0.71 -2.1
249,446 - ECD_00218 0.71 +1.0
249,446 - ECD_00218 0.71 +0.4
249,465 - ECD_00218 0.73 -1.3
249,661 + +0.0
249,662 - -0.0

Or see this region's nucleotide sequence