Strain Fitness in Escherichia coli BL21 around ECD_00200

Experiment: EV152

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntECD_r00002 and ECD_r00003 are separated by 188 nucleotidesECD_r00003 and ECD_t00003 are separated by 52 nucleotidesECD_t00003 and ECD_00200 are separated by 162 nucleotidesECD_00200 and ECD_00201 overlap by 4 nucleotides ECD_r00002: ECD_r00002 - 23S ribosomal RNA, at 228,589 to 231,494 _r00002 ECD_r00003: ECD_r00003 - 5S ribosomal RNA, at 231,683 to 231,802 _r00003 ECD_t00003: ECD_t00003 - tRNA-Asp, at 231,855 to 231,931 _t00003 ECD_00200: ECD_00200 - 2,5-diketo-D-gluconate reductase B, at 232,094 to 232,897 _00200 ECD_00201: ECD_00201 - LysR family putative transcriptional regulator, at 232,894 to 233,808 _00201 Position (kb) 232 233Strain fitness (log2 ratio) -2 -1 0 1at 231.934 kb on + strandat 231.934 kb on + strandat 231.937 kb on + strandat 232.362 kb on - strand, within ECD_00200at 232.424 kb on - strand, within ECD_00200at 232.566 kb on - strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.625 kb on + strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.626 kb on - strand, within ECD_00200at 232.646 kb on - strand, within ECD_00200at 232.895 kb on + strandat 233.163 kb on - strand, within ECD_00201at 233.163 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.177 kb on - strand, within ECD_00201at 233.191 kb on - strand, within ECD_00201at 233.191 kb on - strand, within ECD_00201at 233.368 kb on - strand, within ECD_00201at 233.472 kb on + strand, within ECD_00201at 233.545 kb on - strand, within ECD_00201at 233.638 kb on - strand, within ECD_00201at 233.729 kb on - strandat 233.729 kb on - strandat 233.892 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction EV152
remove
231,934 + +0.2
231,934 + +0.0
231,937 + +0.1
232,362 - ECD_00200 0.33 -0.5
232,424 - ECD_00200 0.41 -0.3
232,566 - ECD_00200 0.59 +1.0
232,625 + ECD_00200 0.66 -0.2
232,625 + ECD_00200 0.66 +1.3
232,625 + ECD_00200 0.66 +0.0
232,626 - ECD_00200 0.66 -1.0
232,626 - ECD_00200 0.66 -2.4
232,646 - ECD_00200 0.69 -0.2
232,895 + -0.2
233,163 - ECD_00201 0.29 +0.5
233,163 - ECD_00201 0.29 -1.1
233,177 - ECD_00201 0.31 +0.3
233,177 - ECD_00201 0.31 +0.5
233,177 - ECD_00201 0.31 -0.8
233,177 - ECD_00201 0.31 -1.3
233,191 - ECD_00201 0.32 +1.0
233,191 - ECD_00201 0.32 +0.8
233,368 - ECD_00201 0.52 +1.0
233,472 + ECD_00201 0.63 -0.3
233,545 - ECD_00201 0.71 +0.7
233,638 - ECD_00201 0.81 -1.4
233,729 - +0.5
233,729 - +1.3
233,892 + -0.1

Or see this region's nucleotide sequence