Experiment: MelonXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapF and LU632_RS24150 overlap by 35 nucleotides LU632_RS24150 and LU632_RS24155 are separated by 164 nucleotides LU632_RS24155 and hemC are separated by 285 nucleotides
LU632_RS24145: dapF - diaminopimelate epimerase, at 4,605,424 to 4,606,248
dapF
LU632_RS24150: LU632_RS24150 - lipoprotein, at 4,606,214 to 4,606,501
_RS24150
LU632_RS24155: LU632_RS24155 - class I adenylate cyclase, at 4,606,666 to 4,609,221
_RS24155
LU632_RS24160: hemC - hydroxymethylbilane synthase, at 4,609,507 to 4,610,448
hemC
Position (kb)
4606
4607
4608
4609
4610 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4606.421 kb on - strand, within LU632_RS24150 at 4606.421 kb on - strand, within LU632_RS24150 at 4606.471 kb on - strand, within LU632_RS24150 at 4606.582 kb on + strand at 4606.583 kb on - strand at 4606.584 kb on + strand at 4606.591 kb on + strand at 4606.592 kb on - strand at 4606.619 kb on - strand at 4606.836 kb on - strand at 4606.840 kb on - strand at 4606.949 kb on + strand, within LU632_RS24155 at 4606.950 kb on - strand, within LU632_RS24155 at 4607.024 kb on - strand, within LU632_RS24155 at 4607.024 kb on - strand, within LU632_RS24155 at 4609.387 kb on - strand at 4609.427 kb on + strand at 4609.465 kb on + strand at 4609.472 kb on + strand at 4609.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MelonXylem remove 4,606,421 - LU632_RS24150 0.72 -2.0 4,606,421 - LU632_RS24150 0.72 +1.8 4,606,471 - LU632_RS24150 0.89 +1.2 4,606,582 + +0.5 4,606,583 - -1.5 4,606,584 + -0.9 4,606,591 + +0.7 4,606,592 - -0.0 4,606,619 - +0.7 4,606,836 - -1.5 4,606,840 - +1.2 4,606,949 + LU632_RS24155 0.11 -1.8 4,606,950 - LU632_RS24155 0.11 +0.7 4,607,024 - LU632_RS24155 0.14 -1.8 4,607,024 - LU632_RS24155 0.14 -2.2 4,609,387 - +0.2 4,609,427 + +0.2 4,609,465 + -0.0 4,609,472 + -0.5 4,609,484 + +0.3
Or see this region's nucleotide sequence