Experiment: MelonXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 109 nucleotides proS and arfB are separated by 389 nucleotides arfB and LU632_RS19855 overlap by 4 nucleotides
LU632_RS19835: rcsF - Rcs stress response system protein RcsF, at 3,769,664 to 3,770,068
rcsF
LU632_RS19840: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 3,770,065 to 3,770,772
tsaA
LU632_RS19845: proS - proline--tRNA ligase, at 3,770,882 to 3,772,600
proS
LU632_RS19850: arfB - aminoacyl-tRNA hydrolase, at 3,772,990 to 3,773,409
arfB
LU632_RS19855: LU632_RS19855 - YaeQ family protein, at 3,773,406 to 3,773,960
_RS19855
Position (kb)
3770
3771
3772
3773 Strain fitness (log2 ratio)
-1
0
1 at 3769.921 kb on + strand, within rcsF at 3769.958 kb on - strand, within rcsF at 3769.958 kb on - strand, within rcsF at 3769.995 kb on + strand, within rcsF at 3769.996 kb on - strand, within rcsF at 3770.148 kb on + strand, within tsaA at 3770.177 kb on + strand, within tsaA at 3770.177 kb on + strand, within tsaA at 3770.188 kb on + strand, within tsaA at 3770.188 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.232 kb on + strand, within tsaA at 3770.233 kb on - strand, within tsaA at 3770.233 kb on - strand, within tsaA at 3770.233 kb on - strand, within tsaA at 3770.233 kb on - strand, within tsaA at 3770.233 kb on - strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.234 kb on + strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.235 kb on - strand, within tsaA at 3770.263 kb on - strand, within tsaA at 3770.322 kb on - strand, within tsaA at 3770.469 kb on + strand, within tsaA at 3770.476 kb on + strand, within tsaA at 3770.535 kb on + strand, within tsaA at 3770.535 kb on + strand, within tsaA at 3770.535 kb on + strand, within tsaA at 3770.535 kb on + strand, within tsaA at 3770.535 kb on + strand, within tsaA at 3770.536 kb on - strand, within tsaA at 3770.536 kb on - strand, within tsaA at 3770.536 kb on - strand, within tsaA at 3770.536 kb on - strand, within tsaA at 3770.623 kb on - strand, within tsaA at 3770.659 kb on + strand, within tsaA at 3770.812 kb on + strand at 3770.812 kb on + strand at 3772.604 kb on + strand at 3772.605 kb on - strand at 3772.635 kb on + strand at 3772.636 kb on - strand at 3772.678 kb on + strand at 3772.706 kb on + strand at 3772.707 kb on - strand at 3772.816 kb on + strand at 3772.816 kb on + strand at 3772.816 kb on + strand at 3772.817 kb on - strand at 3772.817 kb on - strand at 3772.827 kb on - strand at 3773.197 kb on - strand, within arfB at 3773.316 kb on + strand, within arfB at 3773.320 kb on - strand, within arfB at 3773.445 kb on + strand at 3773.459 kb on + strand at 3773.597 kb on + strand, within LU632_RS19855 at 3773.598 kb on - strand, within LU632_RS19855 at 3773.598 kb on - strand, within LU632_RS19855
Per-strain Table
Position Strand Gene LocusTag Fraction MelonXylem remove 3,769,921 + rcsF LU632_RS19835 0.63 +0.2 3,769,958 - rcsF LU632_RS19835 0.73 -1.1 3,769,958 - rcsF LU632_RS19835 0.73 +0.3 3,769,995 + rcsF LU632_RS19835 0.82 +1.0 3,769,996 - rcsF LU632_RS19835 0.82 +0.7 3,770,148 + tsaA LU632_RS19840 0.12 -0.6 3,770,177 + tsaA LU632_RS19840 0.16 +0.4 3,770,177 + tsaA LU632_RS19840 0.16 +0.9 3,770,188 + tsaA LU632_RS19840 0.17 +0.3 3,770,188 + tsaA LU632_RS19840 0.17 +0.1 3,770,232 + tsaA LU632_RS19840 0.24 -0.6 3,770,232 + tsaA LU632_RS19840 0.24 -0.4 3,770,232 + tsaA LU632_RS19840 0.24 -1.0 3,770,232 + tsaA LU632_RS19840 0.24 +0.5 3,770,232 + tsaA LU632_RS19840 0.24 -0.3 3,770,232 + tsaA LU632_RS19840 0.24 -0.6 3,770,232 + tsaA LU632_RS19840 0.24 -0.1 3,770,232 + tsaA LU632_RS19840 0.24 +1.0 3,770,233 - tsaA LU632_RS19840 0.24 +0.2 3,770,233 - tsaA LU632_RS19840 0.24 -0.8 3,770,233 - tsaA LU632_RS19840 0.24 -0.2 3,770,233 - tsaA LU632_RS19840 0.24 -0.0 3,770,233 - tsaA LU632_RS19840 0.24 -0.3 3,770,234 + tsaA LU632_RS19840 0.24 +0.7 3,770,234 + tsaA LU632_RS19840 0.24 +0.0 3,770,234 + tsaA LU632_RS19840 0.24 +0.4 3,770,234 + tsaA LU632_RS19840 0.24 -1.6 3,770,234 + tsaA LU632_RS19840 0.24 +0.1 3,770,234 + tsaA LU632_RS19840 0.24 +0.5 3,770,234 + tsaA LU632_RS19840 0.24 +0.4 3,770,235 - tsaA LU632_RS19840 0.24 -0.6 3,770,235 - tsaA LU632_RS19840 0.24 -0.5 3,770,235 - tsaA LU632_RS19840 0.24 +0.0 3,770,235 - tsaA LU632_RS19840 0.24 +1.0 3,770,235 - tsaA LU632_RS19840 0.24 -0.0 3,770,235 - tsaA LU632_RS19840 0.24 -0.4 3,770,235 - tsaA LU632_RS19840 0.24 +0.9 3,770,235 - tsaA LU632_RS19840 0.24 -1.4 3,770,263 - tsaA LU632_RS19840 0.28 -0.5 3,770,322 - tsaA LU632_RS19840 0.36 +0.2 3,770,469 + tsaA LU632_RS19840 0.57 -0.4 3,770,476 + tsaA LU632_RS19840 0.58 -0.2 3,770,535 + tsaA LU632_RS19840 0.66 -1.6 3,770,535 + tsaA LU632_RS19840 0.66 -0.2 3,770,535 + tsaA LU632_RS19840 0.66 +0.0 3,770,535 + tsaA LU632_RS19840 0.66 -0.8 3,770,535 + tsaA LU632_RS19840 0.66 +0.4 3,770,536 - tsaA LU632_RS19840 0.67 -0.4 3,770,536 - tsaA LU632_RS19840 0.67 -0.4 3,770,536 - tsaA LU632_RS19840 0.67 -0.5 3,770,536 - tsaA LU632_RS19840 0.67 -0.5 3,770,623 - tsaA LU632_RS19840 0.79 -0.2 3,770,659 + tsaA LU632_RS19840 0.84 +0.2 3,770,812 + -0.8 3,770,812 + +0.3 3,772,604 + -0.8 3,772,605 - -1.0 3,772,635 + -0.1 3,772,636 - -0.3 3,772,678 + +0.9 3,772,706 + +0.8 3,772,707 - -0.5 3,772,816 + +0.4 3,772,816 + +1.0 3,772,816 + +0.0 3,772,817 - -0.4 3,772,817 - +0.6 3,772,827 - -0.6 3,773,197 - arfB LU632_RS19850 0.49 +0.3 3,773,316 + arfB LU632_RS19850 0.78 -0.2 3,773,320 - arfB LU632_RS19850 0.79 -0.6 3,773,445 + +0.7 3,773,459 + -0.6 3,773,597 + LU632_RS19855 0.34 -0.1 3,773,598 - LU632_RS19855 0.35 -1.0 3,773,598 - LU632_RS19855 0.35 +0.1
Or see this region's nucleotide sequence