Experiment: MelonXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and gpt are separated by 252 nucleotides gpt and frsA are separated by 131 nucleotides frsA and crl are separated by 104 nucleotides crl and LU632_RS01175 are separated by 79 nucleotides LU632_RS01175 and LU632_RS01180 are separated by 48 nucleotides
LU632_RS01155: pepD - beta-Ala-His dipeptidase, at 245,416 to 246,873
pepD
LU632_RS01160: gpt - xanthine phosphoribosyltransferase, at 247,126 to 247,584
gpt
LU632_RS01165: frsA - esterase FrsA, at 247,716 to 248,948
frsA
LU632_RS01170: crl - sigma factor-binding protein Crl, at 249,053 to 249,454
crl
LU632_RS01175: LU632_RS01175 - porin, at 249,534 to 249,732
_RS01175
LU632_RS01180: LU632_RS01180 - hypothetical protein, at 249,781 to 249,870
_RS01180
Position (kb) 247
248
249 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 246.723 kb on + strand, within pepD at 246.724 kb on - strand, within pepD at 246.768 kb on + strand at 246.768 kb on + strand at 246.771 kb on - strand at 246.771 kb on - strand at 246.876 kb on + strand at 246.887 kb on + strand at 246.888 kb on - strand at 246.982 kb on - strand at 246.999 kb on - strand at 247.061 kb on + strand at 247.229 kb on + strand, within gpt at 247.298 kb on + strand, within gpt at 247.435 kb on + strand, within gpt at 247.560 kb on + strand at 247.667 kb on + strand at 247.667 kb on + strand at 247.667 kb on - strand at 247.697 kb on + strand at 247.698 kb on - strand at 247.702 kb on + strand at 247.703 kb on - strand at 247.703 kb on - strand at 247.734 kb on - strand at 247.806 kb on + strand at 247.806 kb on + strand at 247.807 kb on - strand at 247.862 kb on - strand, within frsA at 247.919 kb on - strand, within frsA at 248.153 kb on - strand, within frsA at 248.169 kb on + strand, within frsA at 248.245 kb on - strand, within frsA at 248.390 kb on + strand, within frsA at 248.390 kb on + strand, within frsA at 248.588 kb on + strand, within frsA at 248.652 kb on + strand, within frsA at 248.652 kb on + strand, within frsA at 248.652 kb on + strand, within frsA at 248.653 kb on - strand, within frsA at 248.654 kb on + strand, within frsA at 248.714 kb on - strand, within frsA at 248.719 kb on + strand, within frsA at 248.720 kb on - strand, within frsA at 248.720 kb on - strand, within frsA at 248.788 kb on - strand, within frsA at 248.788 kb on - strand, within frsA at 248.887 kb on + strand at 248.942 kb on - strand at 249.033 kb on + strand at 249.089 kb on - strand at 249.130 kb on + strand, within crl at 249.183 kb on + strand, within crl at 249.257 kb on + strand, within crl at 249.258 kb on - strand, within crl at 249.263 kb on + strand, within crl at 249.263 kb on + strand, within crl at 249.264 kb on - strand, within crl at 249.267 kb on - strand, within crl at 249.279 kb on - strand, within crl at 249.355 kb on + strand, within crl at 249.355 kb on + strand, within crl at 249.356 kb on - strand, within crl at 249.356 kb on - strand, within crl at 249.401 kb on - strand, within crl at 249.509 kb on + strand at 249.510 kb on - strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.528 kb on - strand at 249.652 kb on + strand, within LU632_RS01175 at 249.653 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.687 kb on - strand, within LU632_RS01175 at 249.731 kb on + strand at 249.785 kb on + strand at 249.785 kb on + strand at 249.810 kb on + strand, within LU632_RS01180
Per-strain Table
Position Strand Gene LocusTag Fraction MelonXylem remove 246,723 + pepD LU632_RS01155 0.90 -0.1 246,724 - pepD LU632_RS01155 0.90 -0.0 246,768 + -0.3 246,768 + -0.5 246,771 - +0.6 246,771 - -0.1 246,876 + +0.1 246,887 + +1.2 246,888 - +1.2 246,982 - +3.2 246,999 - +0.8 247,061 + -1.2 247,229 + gpt LU632_RS01160 0.22 -0.1 247,298 + gpt LU632_RS01160 0.37 -0.6 247,435 + gpt LU632_RS01160 0.67 +0.2 247,560 + +0.6 247,667 + -0.7 247,667 + -0.5 247,667 - -0.2 247,697 + +2.2 247,698 - -0.4 247,702 + -0.6 247,703 - +0.4 247,703 - -0.2 247,734 - +0.4 247,806 + +1.6 247,806 + +0.3 247,807 - +0.3 247,862 - frsA LU632_RS01165 0.12 -0.3 247,919 - frsA LU632_RS01165 0.16 +0.9 248,153 - frsA LU632_RS01165 0.35 -1.0 248,169 + frsA LU632_RS01165 0.37 +0.6 248,245 - frsA LU632_RS01165 0.43 -0.3 248,390 + frsA LU632_RS01165 0.55 -0.4 248,390 + frsA LU632_RS01165 0.55 +0.1 248,588 + frsA LU632_RS01165 0.71 +0.2 248,652 + frsA LU632_RS01165 0.76 +0.3 248,652 + frsA LU632_RS01165 0.76 -2.7 248,652 + frsA LU632_RS01165 0.76 +0.6 248,653 - frsA LU632_RS01165 0.76 -0.1 248,654 + frsA LU632_RS01165 0.76 +0.7 248,714 - frsA LU632_RS01165 0.81 -0.6 248,719 + frsA LU632_RS01165 0.81 -0.5 248,720 - frsA LU632_RS01165 0.81 -0.6 248,720 - frsA LU632_RS01165 0.81 +0.2 248,788 - frsA LU632_RS01165 0.87 +1.4 248,788 - frsA LU632_RS01165 0.87 -0.1 248,887 + +0.6 248,942 - +1.2 249,033 + -0.3 249,089 - -0.4 249,130 + crl LU632_RS01170 0.19 -0.4 249,183 + crl LU632_RS01170 0.32 -0.6 249,257 + crl LU632_RS01170 0.51 +0.2 249,258 - crl LU632_RS01170 0.51 +0.1 249,263 + crl LU632_RS01170 0.52 -0.7 249,263 + crl LU632_RS01170 0.52 +1.1 249,264 - crl LU632_RS01170 0.52 +1.2 249,267 - crl LU632_RS01170 0.53 +0.3 249,279 - crl LU632_RS01170 0.56 +0.2 249,355 + crl LU632_RS01170 0.75 -1.4 249,355 + crl LU632_RS01170 0.75 +1.6 249,356 - crl LU632_RS01170 0.75 +0.6 249,356 - crl LU632_RS01170 0.75 +0.3 249,401 - crl LU632_RS01170 0.87 -0.3 249,509 + -0.2 249,510 - -0.5 249,519 + +0.4 249,519 + +0.4 249,519 + -1.0 249,519 + +0.6 249,520 - -1.4 249,520 - +0.3 249,520 - +0.2 249,520 - -0.0 249,528 - -0.1 249,652 + LU632_RS01175 0.59 -0.8 249,653 - LU632_RS01175 0.60 -0.4 249,656 - LU632_RS01175 0.61 +0.4 249,656 - LU632_RS01175 0.61 +0.1 249,656 - LU632_RS01175 0.61 +0.2 249,676 + LU632_RS01175 0.71 -0.0 249,676 + LU632_RS01175 0.71 -0.9 249,676 + LU632_RS01175 0.71 +1.2 249,677 - LU632_RS01175 0.72 -0.1 249,677 - LU632_RS01175 0.72 +0.2 249,677 - LU632_RS01175 0.72 +0.2 249,687 - LU632_RS01175 0.77 -0.3 249,731 + +0.2 249,785 + +0.4 249,785 + +0.2 249,810 + LU632_RS01180 0.32 +1.0
Or see this region's nucleotide sequence