Experiment: MelonXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdh and argR are separated by 415 nucleotides argR and LU632_RS21375 are separated by 406 nucleotides LU632_RS21375 and LU632_RS21380 are separated by 79 nucleotides LU632_RS21380 and LU632_RS21385 are separated by 16 nucleotides
LU632_RS21365: mdh - malate dehydrogenase, at 4,084,730 to 4,085,665
mdh
LU632_RS21370: argR - transcriptional regulator ArgR, at 4,086,081 to 4,086,551
argR
LU632_RS21375: LU632_RS21375 - DUF1471 domain-containing protein, at 4,086,958 to 4,087,149
_RS21375
LU632_RS21380: LU632_RS21380 - barnase inhibitor, at 4,087,229 to 4,087,498
_RS21380
LU632_RS21385: LU632_RS21385 - aldehyde dehydrogenase family protein, at 4,087,515 to 4,087,688
_RS21385
Position (kb)
4086
4087 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4085.090 kb on + strand, within mdh at 4085.201 kb on - strand, within mdh at 4085.274 kb on - strand, within mdh at 4085.300 kb on - strand, within mdh at 4085.344 kb on + strand, within mdh at 4085.344 kb on + strand, within mdh at 4085.344 kb on + strand, within mdh at 4085.368 kb on + strand, within mdh at 4085.387 kb on - strand, within mdh at 4085.397 kb on - strand, within mdh at 4085.476 kb on + strand, within mdh at 4085.501 kb on - strand, within mdh at 4085.563 kb on + strand, within mdh at 4085.669 kb on - strand at 4085.681 kb on - strand at 4085.681 kb on - strand at 4085.682 kb on + strand at 4085.683 kb on - strand at 4085.683 kb on - strand at 4085.685 kb on + strand at 4085.685 kb on + strand at 4085.788 kb on + strand at 4085.789 kb on - strand at 4085.789 kb on - strand at 4085.814 kb on - strand at 4085.816 kb on + strand at 4085.855 kb on - strand at 4085.915 kb on - strand at 4086.032 kb on - strand at 4086.279 kb on + strand, within argR at 4086.488 kb on - strand, within argR at 4086.548 kb on - strand at 4086.581 kb on + strand at 4086.582 kb on - strand at 4086.623 kb on + strand at 4086.623 kb on - strand at 4086.662 kb on + strand at 4086.663 kb on - strand at 4086.663 kb on - strand at 4086.708 kb on + strand at 4086.755 kb on - strand at 4086.763 kb on + strand at 4086.764 kb on - strand at 4086.947 kb on + strand at 4086.952 kb on + strand at 4086.953 kb on - strand at 4086.954 kb on + strand at 4086.954 kb on + strand at 4086.984 kb on + strand, within LU632_RS21375 at 4087.020 kb on - strand, within LU632_RS21375 at 4087.106 kb on - strand, within LU632_RS21375 at 4087.106 kb on - strand, within LU632_RS21375 at 4087.106 kb on - strand, within LU632_RS21375 at 4087.107 kb on + strand, within LU632_RS21375 at 4087.108 kb on - strand, within LU632_RS21375 at 4087.120 kb on + strand, within LU632_RS21375 at 4087.121 kb on - strand, within LU632_RS21375 at 4087.353 kb on - strand, within LU632_RS21380 at 4087.354 kb on + strand, within LU632_RS21380 at 4087.354 kb on + strand, within LU632_RS21380 at 4087.355 kb on - strand, within LU632_RS21380 at 4087.355 kb on - strand, within LU632_RS21380 at 4087.355 kb on - strand, within LU632_RS21380 at 4087.374 kb on - strand, within LU632_RS21380 at 4087.374 kb on - strand, within LU632_RS21380 at 4087.436 kb on - strand, within LU632_RS21380 at 4087.499 kb on - strand at 4087.532 kb on + strand at 4087.533 kb on - strand, within LU632_RS21385 at 4087.551 kb on - strand, within LU632_RS21385 at 4087.551 kb on - strand, within LU632_RS21385
Per-strain Table
Position Strand Gene LocusTag Fraction MelonXylem remove 4,085,090 + mdh LU632_RS21365 0.38 -0.5 4,085,201 - mdh LU632_RS21365 0.50 -4.2 4,085,274 - mdh LU632_RS21365 0.58 -2.1 4,085,300 - mdh LU632_RS21365 0.61 -3.3 4,085,344 + mdh LU632_RS21365 0.66 -1.3 4,085,344 + mdh LU632_RS21365 0.66 -2.8 4,085,344 + mdh LU632_RS21365 0.66 -2.7 4,085,368 + mdh LU632_RS21365 0.68 -1.3 4,085,387 - mdh LU632_RS21365 0.70 +0.0 4,085,397 - mdh LU632_RS21365 0.71 -1.4 4,085,476 + mdh LU632_RS21365 0.80 -1.2 4,085,501 - mdh LU632_RS21365 0.82 -1.5 4,085,563 + mdh LU632_RS21365 0.89 +1.0 4,085,669 - -0.3 4,085,681 - +0.3 4,085,681 - +0.4 4,085,682 + -3.2 4,085,683 - -0.4 4,085,683 - -0.4 4,085,685 + -1.8 4,085,685 + -2.8 4,085,788 + -1.5 4,085,789 - -0.4 4,085,789 - +0.4 4,085,814 - +0.2 4,085,816 + -0.4 4,085,855 - +0.5 4,085,915 - +0.8 4,086,032 - -1.3 4,086,279 + argR LU632_RS21370 0.42 +1.0 4,086,488 - argR LU632_RS21370 0.86 +0.0 4,086,548 - -0.2 4,086,581 + +0.4 4,086,582 - +0.1 4,086,623 + +0.3 4,086,623 - +0.6 4,086,662 + +0.7 4,086,663 - -1.0 4,086,663 - +0.7 4,086,708 + -0.1 4,086,755 - +1.0 4,086,763 + +0.4 4,086,764 - -0.4 4,086,947 + +0.4 4,086,952 + -0.3 4,086,953 - -0.2 4,086,954 + -1.5 4,086,954 + +0.0 4,086,984 + LU632_RS21375 0.14 +0.3 4,087,020 - LU632_RS21375 0.32 +0.2 4,087,106 - LU632_RS21375 0.77 -1.3 4,087,106 - LU632_RS21375 0.77 +0.2 4,087,106 - LU632_RS21375 0.77 -0.1 4,087,107 + LU632_RS21375 0.78 -0.7 4,087,108 - LU632_RS21375 0.78 +0.9 4,087,120 + LU632_RS21375 0.84 +0.1 4,087,121 - LU632_RS21375 0.85 -0.0 4,087,353 - LU632_RS21380 0.46 -0.2 4,087,354 + LU632_RS21380 0.46 +0.0 4,087,354 + LU632_RS21380 0.46 +0.2 4,087,355 - LU632_RS21380 0.47 +0.2 4,087,355 - LU632_RS21380 0.47 +0.3 4,087,355 - LU632_RS21380 0.47 -0.0 4,087,374 - LU632_RS21380 0.54 +0.8 4,087,374 - LU632_RS21380 0.54 -0.2 4,087,436 - LU632_RS21380 0.77 +0.0 4,087,499 - +0.7 4,087,532 + -0.8 4,087,533 - LU632_RS21385 0.10 -0.4 4,087,551 - LU632_RS21385 0.21 -0.1 4,087,551 - LU632_RS21385 0.21 -0.3
Or see this region's nucleotide sequence