Experiment: MelonXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sltY and trpR are separated by 53 nucleotides trpR and yjjX overlap by 15 nucleotides yjjX and gpmB are separated by 51 nucleotides gpmB and robA are separated by 69 nucleotides
LU632_RS02575: sltY - murein transglycosylase, at 513,039 to 514,964
sltY
LU632_RS02580: trpR - trp operon repressor, at 515,018 to 515,347
trpR
LU632_RS02585: yjjX - inosine/xanthosine triphosphatase, at 515,333 to 515,865
yjjX
LU632_RS02590: gpmB - 2,3-diphosphoglycerate-dependent phosphoglycerate mutase GpmB, at 515,917 to 516,564
gpmB
LU632_RS02595: robA - MDR efflux pump AcrAB transcriptional activator RobA, at 516,634 to 517,524
robA
Position (kb)
515
516
517 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 515.017 kb on + strand at 515.100 kb on - strand, within trpR at 515.127 kb on + strand, within trpR at 515.128 kb on - strand, within trpR at 515.193 kb on + strand, within trpR at 515.194 kb on - strand, within trpR at 515.326 kb on - strand at 515.350 kb on + strand at 515.351 kb on - strand at 515.351 kb on - strand at 515.351 kb on - strand at 515.351 kb on - strand at 515.406 kb on + strand, within yjjX at 515.467 kb on + strand, within yjjX at 515.550 kb on - strand, within yjjX at 515.597 kb on + strand, within yjjX at 515.597 kb on + strand, within yjjX at 515.641 kb on + strand, within yjjX at 515.642 kb on - strand, within yjjX at 515.744 kb on - strand, within yjjX at 515.815 kb on + strand at 515.865 kb on + strand at 515.866 kb on - strand at 515.882 kb on + strand at 515.883 kb on - strand at 515.900 kb on + strand at 515.929 kb on + strand at 516.070 kb on - strand, within gpmB at 516.070 kb on - strand, within gpmB at 516.099 kb on + strand, within gpmB at 516.328 kb on + strand, within gpmB at 516.342 kb on + strand, within gpmB at 516.369 kb on + strand, within gpmB at 516.371 kb on + strand, within gpmB at 516.394 kb on - strand, within gpmB at 516.394 kb on - strand, within gpmB at 516.408 kb on - strand, within gpmB at 516.432 kb on - strand, within gpmB at 516.442 kb on - strand, within gpmB at 516.635 kb on + strand at 516.636 kb on - strand at 516.765 kb on + strand, within robA at 516.765 kb on + strand, within robA at 516.839 kb on + strand, within robA at 516.840 kb on - strand, within robA at 516.840 kb on - strand, within robA at 516.843 kb on + strand, within robA at 516.931 kb on - strand, within robA at 516.932 kb on + strand, within robA at 516.989 kb on + strand, within robA at 516.991 kb on + strand, within robA at 516.991 kb on + strand, within robA at 516.991 kb on + strand, within robA at 516.992 kb on - strand, within robA at 517.005 kb on + strand, within robA at 517.136 kb on + strand, within robA at 517.136 kb on + strand, within robA at 517.136 kb on + strand, within robA at 517.137 kb on - strand, within robA at 517.137 kb on - strand, within robA at 517.137 kb on - strand, within robA at 517.137 kb on - strand, within robA at 517.137 kb on - strand, within robA at 517.365 kb on + strand, within robA at 517.367 kb on + strand, within robA at 517.412 kb on - strand, within robA at 517.412 kb on - strand, within robA at 517.540 kb on + strand at 517.540 kb on + strand at 517.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MelonXylem remove 515,017 + -0.5 515,100 - trpR LU632_RS02580 0.25 -0.6 515,127 + trpR LU632_RS02580 0.33 +1.6 515,128 - trpR LU632_RS02580 0.33 -0.6 515,193 + trpR LU632_RS02580 0.53 +0.4 515,194 - trpR LU632_RS02580 0.53 +0.0 515,326 - -1.3 515,350 + -0.1 515,351 - +0.0 515,351 - +0.1 515,351 - -1.0 515,351 - +0.9 515,406 + yjjX LU632_RS02585 0.14 -0.1 515,467 + yjjX LU632_RS02585 0.25 +0.9 515,550 - yjjX LU632_RS02585 0.41 +0.5 515,597 + yjjX LU632_RS02585 0.50 +0.3 515,597 + yjjX LU632_RS02585 0.50 +0.0 515,641 + yjjX LU632_RS02585 0.58 +0.3 515,642 - yjjX LU632_RS02585 0.58 +1.4 515,744 - yjjX LU632_RS02585 0.77 -0.9 515,815 + +0.3 515,865 + -0.5 515,866 - -0.1 515,882 + +0.3 515,883 - +0.6 515,900 + +0.0 515,929 + +0.8 516,070 - gpmB LU632_RS02590 0.24 -0.4 516,070 - gpmB LU632_RS02590 0.24 -0.6 516,099 + gpmB LU632_RS02590 0.28 -0.3 516,328 + gpmB LU632_RS02590 0.63 -1.5 516,342 + gpmB LU632_RS02590 0.66 -1.1 516,369 + gpmB LU632_RS02590 0.70 +0.0 516,371 + gpmB LU632_RS02590 0.70 +0.0 516,394 - gpmB LU632_RS02590 0.74 +0.4 516,394 - gpmB LU632_RS02590 0.74 -0.8 516,408 - gpmB LU632_RS02590 0.76 -0.2 516,432 - gpmB LU632_RS02590 0.79 -1.8 516,442 - gpmB LU632_RS02590 0.81 +0.6 516,635 + +1.0 516,636 - -0.8 516,765 + robA LU632_RS02595 0.15 -0.6 516,765 + robA LU632_RS02595 0.15 +0.0 516,839 + robA LU632_RS02595 0.23 +0.5 516,840 - robA LU632_RS02595 0.23 +1.0 516,840 - robA LU632_RS02595 0.23 -1.3 516,843 + robA LU632_RS02595 0.23 +0.4 516,931 - robA LU632_RS02595 0.33 +0.4 516,932 + robA LU632_RS02595 0.33 +2.0 516,989 + robA LU632_RS02595 0.40 -0.3 516,991 + robA LU632_RS02595 0.40 +0.0 516,991 + robA LU632_RS02595 0.40 -0.1 516,991 + robA LU632_RS02595 0.40 -0.3 516,992 - robA LU632_RS02595 0.40 -0.7 517,005 + robA LU632_RS02595 0.42 +0.5 517,136 + robA LU632_RS02595 0.56 +0.6 517,136 + robA LU632_RS02595 0.56 -0.7 517,136 + robA LU632_RS02595 0.56 +1.6 517,137 - robA LU632_RS02595 0.56 -1.6 517,137 - robA LU632_RS02595 0.56 +0.8 517,137 - robA LU632_RS02595 0.56 +3.0 517,137 - robA LU632_RS02595 0.56 +0.4 517,137 - robA LU632_RS02595 0.56 -0.6 517,365 + robA LU632_RS02595 0.82 +0.7 517,367 + robA LU632_RS02595 0.82 +0.4 517,412 - robA LU632_RS02595 0.87 -2.0 517,412 - robA LU632_RS02595 0.87 -0.4 517,540 + -1.2 517,540 + +0.4 517,541 - -0.5
Or see this region's nucleotide sequence