Strain Fitness in Escherichia coli BL21 around ECD_01018

Experiment: Laval1257

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01017 and ECD_01018 are separated by 422 nucleotidesECD_01018 and ECD_01019 are separated by 533 nucleotides ECD_01017: ECD_01017 - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 1,079,202 to 1,083,164 _01017 ECD_01018: ECD_01018 - proline:sodium symporter, at 1,083,587 to 1,085,095 _01018 ECD_01019: ECD_01019 - putative membrane protein, at 1,085,629 to 1,086,468 _01019 Position (kb) 1083 1084 1085 1086Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1082.589 kb on - strand, within ECD_01017at 1082.643 kb on - strand, within ECD_01017at 1082.713 kb on - strand, within ECD_01017at 1083.065 kb on + strandat 1083.171 kb on - strandat 1083.171 kb on - strandat 1083.539 kb on + strandat 1083.595 kb on + strandat 1083.626 kb on + strandat 1083.642 kb on + strandat 1083.689 kb on + strandat 1083.691 kb on + strandat 1083.691 kb on + strandat 1083.691 kb on + strandat 1084.152 kb on + strand, within ECD_01018at 1084.270 kb on + strand, within ECD_01018at 1084.360 kb on + strand, within ECD_01018at 1084.612 kb on - strand, within ECD_01018at 1084.656 kb on + strand, within ECD_01018at 1084.963 kb on + strandat 1084.993 kb on + strandat 1085.057 kb on + strandat 1085.283 kb on - strandat 1085.337 kb on + strandat 1085.338 kb on - strandat 1085.602 kb on + strandat 1085.658 kb on + strandat 1085.659 kb on - strandat 1085.710 kb on + strandat 1085.710 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1257
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1,082,589 - ECD_01017 0.85 -2.8
1,082,643 - ECD_01017 0.87 -0.2
1,082,713 - ECD_01017 0.89 -1.2
1,083,065 + +1.3
1,083,171 - -0.1
1,083,171 - +1.1
1,083,539 + +1.0
1,083,595 + +0.4
1,083,626 + +1.1
1,083,642 + -0.3
1,083,689 + +0.4
1,083,691 + +1.2
1,083,691 + -0.8
1,083,691 + -0.6
1,084,152 + ECD_01018 0.37 +0.0
1,084,270 + ECD_01018 0.45 +1.2
1,084,360 + ECD_01018 0.51 +0.0
1,084,612 - ECD_01018 0.68 +1.3
1,084,656 + ECD_01018 0.71 -1.2
1,084,963 + +0.5
1,084,993 + +1.1
1,085,057 + -0.5
1,085,283 - +0.3
1,085,337 + -0.2
1,085,338 - -1.4
1,085,602 + +0.8
1,085,658 + +0.3
1,085,659 - +2.3
1,085,710 + +1.5
1,085,710 + +1.5

Or see this region's nucleotide sequence