Strain Fitness in Escherichia coli BL21 around ECD_00968

Experiment: Laval1257

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00966 and ECD_00967 are separated by 31 nucleotidesECD_00967 and ECD_00968 are separated by 95 nucleotidesECD_00968 and ECD_00969 are separated by 172 nucleotidesECD_00969 and ECD_00970 are separated by 44 nucleotides ECD_00966: ECD_00966 - DNA helicase IV, at 1,029,560 to 1,031,614 _00966 ECD_00967: ECD_00967 - methylglyoxal synthase, at 1,031,646 to 1,032,104 _00967 ECD_00968: ECD_00968 - UPF0319 family protein, at 1,032,200 to 1,032,862 _00968 ECD_00969: ECD_00969 - putative CoA-binding protein, at 1,033,035 to 1,033,448 _00969 ECD_00970: ECD_00970 - heat shock protein involved in degradation of mutant DnaA; hemimethylated oriC DNA-binding protein, at 1,033,493 to 1,033,810 _00970 Position (kb) 1032 1033Strain fitness (log2 ratio) -2 -1 0 1at 1031.498 kb on + strandat 1031.957 kb on + strand, within ECD_00967at 1031.977 kb on + strand, within ECD_00967at 1031.983 kb on - strand, within ECD_00967at 1031.983 kb on - strand, within ECD_00967at 1032.066 kb on - strandat 1032.082 kb on - strandat 1032.110 kb on - strandat 1032.117 kb on + strandat 1032.143 kb on - strandat 1032.143 kb on - strandat 1032.349 kb on + strand, within ECD_00968at 1032.632 kb on - strand, within ECD_00968at 1032.632 kb on - strand, within ECD_00968at 1032.633 kb on + strand, within ECD_00968at 1032.633 kb on + strand, within ECD_00968at 1032.634 kb on - strand, within ECD_00968at 1032.634 kb on - strand, within ECD_00968at 1032.974 kb on + strandat 1033.137 kb on + strand, within ECD_00969at 1033.304 kb on + strand, within ECD_00969at 1033.495 kb on - strandat 1033.593 kb on - strand, within ECD_00970at 1033.593 kb on - strand, within ECD_00970at 1033.593 kb on - strand, within ECD_00970at 1033.593 kb on - strand, within ECD_00970at 1033.842 kb on - strandat 1033.842 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1257
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1,031,498 + -0.9
1,031,957 + ECD_00967 0.68 +0.5
1,031,977 + ECD_00967 0.72 -0.8
1,031,983 - ECD_00967 0.73 +0.9
1,031,983 - ECD_00967 0.73 +0.6
1,032,066 - -0.1
1,032,082 - +1.4
1,032,110 - -0.3
1,032,117 + -1.8
1,032,143 - +0.7
1,032,143 - -0.6
1,032,349 + ECD_00968 0.22 +1.1
1,032,632 - ECD_00968 0.65 -2.2
1,032,632 - ECD_00968 0.65 -0.7
1,032,633 + ECD_00968 0.65 -0.4
1,032,633 + ECD_00968 0.65 -1.6
1,032,634 - ECD_00968 0.65 -1.0
1,032,634 - ECD_00968 0.65 -0.7
1,032,974 + +0.3
1,033,137 + ECD_00969 0.25 +0.1
1,033,304 + ECD_00969 0.65 -0.2
1,033,495 - -0.5
1,033,593 - ECD_00970 0.31 -0.9
1,033,593 - ECD_00970 0.31 -0.1
1,033,593 - ECD_00970 0.31 +0.6
1,033,593 - ECD_00970 0.31 -0.4
1,033,842 - +0.2
1,033,842 - +0.2

Or see this region's nucleotide sequence