Experiment: CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 15 nucleotides fruK and fruB overlap by 4 nucleotides fruB and LU632_RS17330 are separated by 276 nucleotides LU632_RS17330 and LU632_RS17335 are separated by 66 nucleotides
LU632_RS17315: fruA - PTS fructose transporter subunit IIBC, at 3,254,161 to 3,255,861
fruA
LU632_RS17320: fruK - 1-phosphofructokinase, at 3,255,877 to 3,256,815
fruK
LU632_RS17325: fruB - fused PTS fructose transporter subunit IIA/HPr protein, at 3,256,812 to 3,257,945
fruB
LU632_RS17330: LU632_RS17330 - site-specific integrase, at 3,258,222 to 3,258,755
_RS17330
LU632_RS17335: LU632_RS17335 - IS6 family transposase, at 3,258,822 to 3,259,526
_RS17335
Position (kb)
3256
3257
3258 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3255.844 kb on + strand at 3255.845 kb on - strand at 3255.862 kb on + strand at 3255.862 kb on + strand at 3255.863 kb on - strand at 3255.863 kb on - strand at 3255.882 kb on + strand at 3255.892 kb on + strand at 3255.892 kb on + strand at 3255.892 kb on + strand at 3256.028 kb on + strand, within fruK at 3256.201 kb on + strand, within fruK at 3256.201 kb on + strand, within fruK at 3256.202 kb on - strand, within fruK at 3256.323 kb on + strand, within fruK at 3256.324 kb on - strand, within fruK at 3256.548 kb on + strand, within fruK at 3256.549 kb on - strand, within fruK at 3257.016 kb on - strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.179 kb on + strand, within fruB at 3257.180 kb on - strand, within fruB at 3257.180 kb on - strand, within fruB at 3257.182 kb on - strand, within fruB at 3257.198 kb on - strand, within fruB at 3257.198 kb on - strand, within fruB at 3257.240 kb on + strand, within fruB at 3257.477 kb on + strand, within fruB at 3257.606 kb on + strand, within fruB at 3257.607 kb on - strand, within fruB at 3257.650 kb on - strand, within fruB at 3257.665 kb on + strand, within fruB at 3257.726 kb on + strand, within fruB at 3257.726 kb on + strand, within fruB at 3257.730 kb on + strand, within fruB at 3257.788 kb on + strand, within fruB at 3257.788 kb on + strand, within fruB at 3257.789 kb on - strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.792 kb on + strand, within fruB at 3257.830 kb on + strand, within fruB at 3257.831 kb on - strand, within fruB at 3257.837 kb on + strand at 3257.842 kb on - strand at 3257.945 kb on + strand at 3257.945 kb on + strand at 3257.945 kb on + strand at 3257.946 kb on - strand at 3257.946 kb on - strand at 3257.946 kb on - strand at 3257.946 kb on - strand at 3257.946 kb on - strand at 3258.073 kb on - strand at 3258.237 kb on - strand at 3258.241 kb on + strand at 3258.313 kb on - strand, within LU632_RS17330 at 3258.313 kb on - strand, within LU632_RS17330 at 3258.313 kb on - strand, within LU632_RS17330 at 3258.422 kb on - strand, within LU632_RS17330 at 3258.425 kb on + strand, within LU632_RS17330 at 3258.456 kb on + strand, within LU632_RS17330 at 3258.458 kb on + strand, within LU632_RS17330 at 3258.504 kb on + strand, within LU632_RS17330 at 3258.552 kb on - strand, within LU632_RS17330 at 3258.642 kb on + strand, within LU632_RS17330 at 3258.642 kb on + strand, within LU632_RS17330 at 3258.643 kb on - strand, within LU632_RS17330 at 3258.646 kb on + strand, within LU632_RS17330 at 3258.647 kb on - strand, within LU632_RS17330 at 3258.690 kb on + strand, within LU632_RS17330
Per-strain Table
Position Strand Gene LocusTag Fraction CucXylem remove 3,255,844 + +0.7 3,255,845 - -0.5 3,255,862 + +0.7 3,255,862 + +0.3 3,255,863 - -0.2 3,255,863 - -1.3 3,255,882 + +0.1 3,255,892 + -1.7 3,255,892 + -3.0 3,255,892 + -2.3 3,256,028 + fruK LU632_RS17320 0.16 -2.2 3,256,201 + fruK LU632_RS17320 0.35 -1.3 3,256,201 + fruK LU632_RS17320 0.35 +0.1 3,256,202 - fruK LU632_RS17320 0.35 +1.3 3,256,323 + fruK LU632_RS17320 0.47 -1.8 3,256,324 - fruK LU632_RS17320 0.48 -0.7 3,256,548 + fruK LU632_RS17320 0.71 -0.9 3,256,549 - fruK LU632_RS17320 0.72 +0.1 3,257,016 - fruB LU632_RS17325 0.18 -1.0 3,257,179 + fruB LU632_RS17325 0.32 -1.0 3,257,179 + fruB LU632_RS17325 0.32 -1.4 3,257,179 + fruB LU632_RS17325 0.32 -0.3 3,257,180 - fruB LU632_RS17325 0.32 -2.3 3,257,180 - fruB LU632_RS17325 0.32 -2.8 3,257,182 - fruB LU632_RS17325 0.33 +0.7 3,257,198 - fruB LU632_RS17325 0.34 -3.3 3,257,198 - fruB LU632_RS17325 0.34 -1.6 3,257,240 + fruB LU632_RS17325 0.38 -1.3 3,257,477 + fruB LU632_RS17325 0.59 -2.0 3,257,606 + fruB LU632_RS17325 0.70 -1.5 3,257,607 - fruB LU632_RS17325 0.70 -0.5 3,257,650 - fruB LU632_RS17325 0.74 -1.3 3,257,665 + fruB LU632_RS17325 0.75 -2.0 3,257,726 + fruB LU632_RS17325 0.81 -2.7 3,257,726 + fruB LU632_RS17325 0.81 +0.4 3,257,730 + fruB LU632_RS17325 0.81 -3.1 3,257,788 + fruB LU632_RS17325 0.86 -0.3 3,257,788 + fruB LU632_RS17325 0.86 -1.3 3,257,789 - fruB LU632_RS17325 0.86 -1.1 3,257,792 + fruB LU632_RS17325 0.86 -1.6 3,257,792 + fruB LU632_RS17325 0.86 -0.9 3,257,792 + fruB LU632_RS17325 0.86 -1.1 3,257,792 + fruB LU632_RS17325 0.86 -0.7 3,257,830 + fruB LU632_RS17325 0.90 -1.2 3,257,831 - fruB LU632_RS17325 0.90 -1.4 3,257,837 + -1.8 3,257,842 - -0.8 3,257,945 + -1.6 3,257,945 + -2.0 3,257,945 + -1.1 3,257,946 - -1.2 3,257,946 - -1.6 3,257,946 - -1.4 3,257,946 - -0.6 3,257,946 - -1.7 3,258,073 - -0.2 3,258,237 - +0.7 3,258,241 + -0.8 3,258,313 - LU632_RS17330 0.17 -0.7 3,258,313 - LU632_RS17330 0.17 -0.5 3,258,313 - LU632_RS17330 0.17 +0.6 3,258,422 - LU632_RS17330 0.37 -1.3 3,258,425 + LU632_RS17330 0.38 -1.3 3,258,456 + LU632_RS17330 0.44 +0.1 3,258,458 + LU632_RS17330 0.44 -0.1 3,258,504 + LU632_RS17330 0.53 -0.9 3,258,552 - LU632_RS17330 0.62 -0.7 3,258,642 + LU632_RS17330 0.79 +0.4 3,258,642 + LU632_RS17330 0.79 -0.1 3,258,643 - LU632_RS17330 0.79 -0.2 3,258,646 + LU632_RS17330 0.79 +0.8 3,258,647 - LU632_RS17330 0.80 +0.0 3,258,690 + LU632_RS17330 0.88 +0.7
Or see this region's nucleotide sequence