Strain Fitness in Escherichia coli BL21 around ECD_04049

Experiment: Bas30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_04047 and ECD_04048 are separated by 126 nucleotidesECD_04048 and ECD_04049 are separated by 18 nucleotidesECD_04049 and ECD_04050 are separated by 50 nucleotidesECD_04050 and ECD_04051 are separated by 17 nucleotides ECD_04047: ECD_04047 - 23S rRNA mG2251 2'-O-ribose methyltransferase, SAM-dependent, at 4,315,795 to 4,316,526 _04047 ECD_04048: ECD_04048 - DUF2170 family protein, at 4,316,653 to 4,317,054 _04048 ECD_04049: ECD_04049 - PspA/IM30 family protein, at 4,317,073 to 4,317,771 _04049 ECD_04050: ECD_04050 - DUF2491 family protein, at 4,317,822 to 4,318,481 _04050 ECD_04051: ECD_04051 - UPF0719 family inner membrane protein, at 4,318,499 to 4,318,897 _04051 Position (kb) 4317 4318Strain fitness (log2 ratio) -1 0 1at 4316.137 kb on - strand, within ECD_04047at 4316.280 kb on + strand, within ECD_04047at 4316.280 kb on + strand, within ECD_04047at 4316.280 kb on + strand, within ECD_04047at 4316.281 kb on - strand, within ECD_04047at 4316.281 kb on - strand, within ECD_04047at 4316.281 kb on - strand, within ECD_04047at 4316.281 kb on - strand, within ECD_04047at 4316.334 kb on + strand, within ECD_04047at 4316.335 kb on - strand, within ECD_04047at 4316.335 kb on - strand, within ECD_04047at 4316.335 kb on - strand, within ECD_04047at 4316.335 kb on - strand, within ECD_04047at 4316.341 kb on + strand, within ECD_04047at 4316.342 kb on - strand, within ECD_04047at 4316.398 kb on - strand, within ECD_04047at 4316.832 kb on + strand, within ECD_04048at 4316.935 kb on + strand, within ECD_04048at 4317.328 kb on - strand, within ECD_04049at 4317.737 kb on + strandat 4317.737 kb on + strandat 4317.741 kb on - strandat 4317.860 kb on + strandat 4318.035 kb on + strand, within ECD_04050at 4318.038 kb on + strand, within ECD_04050at 4318.039 kb on - strand, within ECD_04050at 4318.088 kb on + strand, within ECD_04050at 4318.089 kb on - strand, within ECD_04050at 4318.135 kb on - strand, within ECD_04050at 4318.470 kb on - strandat 4318.671 kb on - strand, within ECD_04051

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas30
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4,316,137 - ECD_04047 0.47 +0.1
4,316,280 + ECD_04047 0.66 +0.1
4,316,280 + ECD_04047 0.66 +0.8
4,316,280 + ECD_04047 0.66 +0.3
4,316,281 - ECD_04047 0.66 -1.5
4,316,281 - ECD_04047 0.66 +0.0
4,316,281 - ECD_04047 0.66 -0.3
4,316,281 - ECD_04047 0.66 -0.7
4,316,334 + ECD_04047 0.74 +0.0
4,316,335 - ECD_04047 0.74 +0.1
4,316,335 - ECD_04047 0.74 +1.3
4,316,335 - ECD_04047 0.74 -0.7
4,316,335 - ECD_04047 0.74 +1.0
4,316,341 + ECD_04047 0.75 +0.2
4,316,342 - ECD_04047 0.75 -0.0
4,316,398 - ECD_04047 0.82 +0.0
4,316,832 + ECD_04048 0.45 +0.9
4,316,935 + ECD_04048 0.70 +0.0
4,317,328 - ECD_04049 0.36 -0.6
4,317,737 + +0.4
4,317,737 + -0.4
4,317,741 - -1.6
4,317,860 + +0.6
4,318,035 + ECD_04050 0.32 -0.2
4,318,038 + ECD_04050 0.33 +0.5
4,318,039 - ECD_04050 0.33 +0.2
4,318,088 + ECD_04050 0.40 +0.4
4,318,089 - ECD_04050 0.40 -0.1
4,318,135 - ECD_04050 0.47 +1.1
4,318,470 - +0.3
4,318,671 - ECD_04051 0.43 -1.0

Or see this region's nucleotide sequence