Strain Fitness in Escherichia coli BL21 around ECD_01049

Experiment: Bas30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01046 and ECD_01047 are separated by 172 nucleotidesECD_01047 and ECD_01048 are separated by 0 nucleotidesECD_01048 and ECD_01049 are separated by 82 nucleotidesECD_01049 and ECD_01050 are separated by 171 nucleotides ECD_01046: ECD_01046 - OPG biosynthetic ACP-dependent transmembrane UDP-glucose beta-1,2 glycosyltransferase; nutrient-dependent cell size regulator, FtsZ assembly antagonist, at 1,113,430 to 1,115,973 _01046 ECD_01047: ECD_01047 - outer membrane integrity lipoprotein, at 1,116,146 to 1,116,373 _01047 ECD_01048: ECD_01048 - multicopy suppressor of secY and secA, at 1,116,374 to 1,116,748 _01048 ECD_01049: ECD_01049 - putative drug efflux system protein, at 1,116,831 to 1,118,057 _01049 ECD_01050: ECD_01050 - lauryl-acyl carrier protein (ACP)-dependent acyltransferase, at 1,118,229 to 1,119,149 _01050 Position (kb) 1116 1117 1118 1119Strain fitness (log2 ratio) -2 -1 0 1at 1116.366 kb on - strandat 1116.465 kb on - strand, within ECD_01048at 1116.470 kb on - strand, within ECD_01048at 1116.495 kb on - strand, within ECD_01048at 1116.739 kb on - strandat 1117.325 kb on - strand, within ECD_01049at 1117.325 kb on - strand, within ECD_01049at 1117.325 kb on - strand, within ECD_01049at 1117.413 kb on - strand, within ECD_01049at 1117.798 kb on - strand, within ECD_01049at 1118.286 kb on - strandat 1118.286 kb on - strandat 1118.286 kb on - strandat 1118.288 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas30
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1,116,366 - +0.8
1,116,465 - ECD_01048 0.24 +0.4
1,116,470 - ECD_01048 0.26 -0.4
1,116,495 - ECD_01048 0.32 +0.6
1,116,739 - +1.3
1,117,325 - ECD_01049 0.40 -0.3
1,117,325 - ECD_01049 0.40 -0.3
1,117,325 - ECD_01049 0.40 -1.8
1,117,413 - ECD_01049 0.47 -0.9
1,117,798 - ECD_01049 0.79 -0.2
1,118,286 - +1.1
1,118,286 - +1.1
1,118,286 - +1.1
1,118,288 - +1.1

Or see this region's nucleotide sequence