Strain Fitness in Escherichia coli BL21 around ECD_00477

Experiment: Bas30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00476 and ECD_00477 are separated by 35 nucleotidesECD_00477 and ECD_00478 are separated by 107 nucleotidesECD_00478 and ECD_00479 are separated by 1 nucleotides ECD_00476: ECD_00476 - cysteinyl-tRNA synthetase, at 523,698 to 525,083 _00476 ECD_00477: ECD_00477 - DUF457 family inner membrane protein, at 525,119 to 525,640 _00477 ECD_00478: ECD_00478 - ribosome-associated protein; putative RNA-binding protein, at 525,748 to 525,960 _00478 ECD_00479: ECD_00479 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase, at 525,962 to 526,828 _00479 Position (kb) 525 526Strain fitness (log2 ratio) -1 0 1at 525.082 kb on - strandat 525.152 kb on + strandat 525.422 kb on + strand, within ECD_00477at 525.422 kb on + strand, within ECD_00477at 525.423 kb on - strand, within ECD_00477at 525.423 kb on - strand, within ECD_00477at 525.606 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas30
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525,082 - -0.2
525,152 + -0.7
525,422 + ECD_00477 0.58 +0.4
525,422 + ECD_00477 0.58 +0.7
525,423 - ECD_00477 0.58 +0.2
525,423 - ECD_00477 0.58 +0.5
525,606 - +0.9

Or see this region's nucleotide sequence