Strain Fitness in Escherichia coli BL21 around ECD_01016

Experiment: Bas50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01015 and ECD_01016 are separated by 230 nucleotidesECD_01016 and ECD_01017 are separated by 39 nucleotides ECD_01015: ECD_01015 - pyrimidine oxygenase, FMN-dependent, at 1,077,145 to 1,078,293 _01015 ECD_01016: ECD_01016 - rut operon transcriptional repressor for, at 1,078,524 to 1,079,162 _01016 ECD_01017: ECD_01017 - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 1,079,202 to 1,083,164 _01017 Position (kb) 1078 1079 1080Strain fitness (log2 ratio) -2 -1 0 1at 1077.797 kb on + strand, within ECD_01015at 1077.830 kb on + strand, within ECD_01015at 1077.967 kb on + strand, within ECD_01015at 1079.182 kb on - strandat 1079.273 kb on - strandat 1079.448 kb on - strandat 1079.482 kb on - strandat 1079.482 kb on - strandat 1079.685 kb on - strand, within ECD_01017at 1079.685 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.689 kb on - strand, within ECD_01017at 1079.934 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.937 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.948 kb on - strand, within ECD_01017at 1079.988 kb on - strand, within ECD_01017

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas50
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1,077,797 + ECD_01015 0.57 -0.2
1,077,830 + ECD_01015 0.60 -0.3
1,077,967 + ECD_01015 0.72 +0.2
1,079,182 - -1.0
1,079,273 - -0.3
1,079,448 - +0.1
1,079,482 - +0.6
1,079,482 - +1.2
1,079,685 - ECD_01017 0.12 -0.7
1,079,685 - ECD_01017 0.12 +0.2
1,079,689 - ECD_01017 0.12 +0.5
1,079,689 - ECD_01017 0.12 -1.3
1,079,689 - ECD_01017 0.12 -0.2
1,079,689 - ECD_01017 0.12 -1.0
1,079,934 - ECD_01017 0.18 +0.4
1,079,937 - ECD_01017 0.19 +1.2
1,079,937 - ECD_01017 0.19 +0.4
1,079,937 - ECD_01017 0.19 -1.8
1,079,948 - ECD_01017 0.19 -0.5
1,079,948 - ECD_01017 0.19 +0.4
1,079,948 - ECD_01017 0.19 +0.6
1,079,988 - ECD_01017 0.20 +0.1

Or see this region's nucleotide sequence