Strain Fitness in Escherichia coli BL21 around ECD_00216

Experiment: Bas50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00214 and ECD_00215 are separated by 153 nucleotidesECD_00215 and ECD_00216 are separated by 42 nucleotidesECD_00216 and ECD_00217 are separated by 239 nucleotides ECD_00214: ECD_00214 - putative NAD(P)-binding C-N hydrolase family amidase, at 243,993 to 244,763 _00214 ECD_00215: ECD_00215 - inhibitor of c-type lysozyme, periplasmic, at 244,917 to 245,390 _00215 ECD_00216: ECD_00216 - acyl coenzyme A dehydrogenase, at 245,433 to 247,877 _00216 ECD_00217: ECD_00217 - D-sedoheptulose 7-phosphate isomerase, at 248,117 to 248,695 _00217 Position (kb) 245 246 247 248Strain fitness (log2 ratio) -2 -1 0 1at 244.446 kb on - strand, within ECD_00214at 244.459 kb on - strand, within ECD_00214at 244.516 kb on - strand, within ECD_00214at 244.516 kb on - strand, within ECD_00214at 244.559 kb on + strand, within ECD_00214at 244.560 kb on - strand, within ECD_00214at 244.560 kb on - strand, within ECD_00214at 244.578 kb on + strand, within ECD_00214at 244.578 kb on + strand, within ECD_00214at 244.578 kb on + strand, within ECD_00214at 244.670 kb on + strand, within ECD_00214at 244.670 kb on + strand, within ECD_00214at 244.671 kb on - strand, within ECD_00214at 244.671 kb on - strand, within ECD_00214at 244.746 kb on - strandat 244.768 kb on + strandat 244.768 kb on + strandat 244.769 kb on - strandat 244.769 kb on - strandat 244.793 kb on + strandat 244.794 kb on - strandat 244.856 kb on + strandat 244.903 kb on - strandat 244.913 kb on + strandat 245.011 kb on + strand, within ECD_00215at 245.068 kb on + strand, within ECD_00215at 245.068 kb on + strand, within ECD_00215at 245.068 kb on + strand, within ECD_00215at 245.069 kb on - strand, within ECD_00215at 245.079 kb on - strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.112 kb on + strand, within ECD_00215at 245.113 kb on - strand, within ECD_00215at 245.113 kb on - strand, within ECD_00215at 245.113 kb on - strand, within ECD_00215at 245.113 kb on - strand, within ECD_00215at 245.113 kb on - strand, within ECD_00215at 245.151 kb on + strand, within ECD_00215at 245.151 kb on + strand, within ECD_00215at 245.198 kb on + strand, within ECD_00215at 245.199 kb on - strand, within ECD_00215at 245.426 kb on + strandat 245.434 kb on + strandat 245.637 kb on - strandat 245.648 kb on + strandat 245.801 kb on + strand, within ECD_00216at 245.929 kb on + strand, within ECD_00216at 245.943 kb on + strand, within ECD_00216at 246.151 kb on + strand, within ECD_00216at 246.152 kb on - strand, within ECD_00216at 246.252 kb on - strand, within ECD_00216at 246.418 kb on + strand, within ECD_00216at 246.521 kb on - strand, within ECD_00216at 246.521 kb on - strand, within ECD_00216at 246.563 kb on + strand, within ECD_00216at 246.575 kb on + strand, within ECD_00216at 246.605 kb on - strand, within ECD_00216at 246.653 kb on + strand, within ECD_00216at 246.654 kb on - strand, within ECD_00216at 246.654 kb on - strand, within ECD_00216at 246.692 kb on - strand, within ECD_00216at 246.692 kb on - strand, within ECD_00216at 246.826 kb on + strand, within ECD_00216at 246.827 kb on - strand, within ECD_00216at 247.037 kb on + strand, within ECD_00216at 247.257 kb on + strand, within ECD_00216at 247.334 kb on + strand, within ECD_00216at 247.337 kb on - strand, within ECD_00216at 247.361 kb on - strand, within ECD_00216at 247.406 kb on - strand, within ECD_00216at 247.534 kb on + strand, within ECD_00216at 247.538 kb on + strand, within ECD_00216at 247.538 kb on + strand, within ECD_00216at 247.539 kb on - strand, within ECD_00216at 247.539 kb on - strand, within ECD_00216at 247.663 kb on - strandat 247.686 kb on - strandat 247.810 kb on - strandat 247.951 kb on - strandat 247.974 kb on - strandat 248.092 kb on + strandat 248.121 kb on - strandat 248.377 kb on + strand, within ECD_00217at 248.776 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas50
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244,446 - ECD_00214 0.59 -1.9
244,459 - ECD_00214 0.60 -2.3
244,516 - ECD_00214 0.68 +0.5
244,516 - ECD_00214 0.68 -0.3
244,559 + ECD_00214 0.73 +0.7
244,560 - ECD_00214 0.74 -1.1
244,560 - ECD_00214 0.74 +0.2
244,578 + ECD_00214 0.76 +0.4
244,578 + ECD_00214 0.76 -0.4
244,578 + ECD_00214 0.76 -0.7
244,670 + ECD_00214 0.88 -1.5
244,670 + ECD_00214 0.88 +0.2
244,671 - ECD_00214 0.88 +0.4
244,671 - ECD_00214 0.88 -1.5
244,746 - +0.1
244,768 + -0.4
244,768 + +0.1
244,769 - +0.5
244,769 - -0.1
244,793 + -0.5
244,794 - -1.0
244,856 + -0.3
244,903 - +0.3
244,913 + +0.1
245,011 + ECD_00215 0.20 -0.9
245,068 + ECD_00215 0.32 -0.4
245,068 + ECD_00215 0.32 -0.1
245,068 + ECD_00215 0.32 +0.3
245,069 - ECD_00215 0.32 +1.0
245,079 - ECD_00215 0.34 +0.2
245,112 + ECD_00215 0.41 -0.6
245,112 + ECD_00215 0.41 -0.4
245,112 + ECD_00215 0.41 +0.8
245,112 + ECD_00215 0.41 +0.3
245,112 + ECD_00215 0.41 -0.4
245,112 + ECD_00215 0.41 +0.2
245,113 - ECD_00215 0.41 +0.4
245,113 - ECD_00215 0.41 -1.0
245,113 - ECD_00215 0.41 -0.9
245,113 - ECD_00215 0.41 -0.0
245,113 - ECD_00215 0.41 -1.5
245,151 + ECD_00215 0.49 +0.5
245,151 + ECD_00215 0.49 -0.4
245,198 + ECD_00215 0.59 +0.2
245,199 - ECD_00215 0.59 +0.9
245,426 + -1.2
245,434 + +1.1
245,637 - -0.0
245,648 + -1.5
245,801 + ECD_00216 0.15 +1.0
245,929 + ECD_00216 0.20 -1.2
245,943 + ECD_00216 0.21 -0.4
246,151 + ECD_00216 0.29 +0.2
246,152 - ECD_00216 0.29 -0.4
246,252 - ECD_00216 0.33 +0.4
246,418 + ECD_00216 0.40 +1.1
246,521 - ECD_00216 0.44 +0.3
246,521 - ECD_00216 0.44 +0.4
246,563 + ECD_00216 0.46 +1.3
246,575 + ECD_00216 0.47 +0.1
246,605 - ECD_00216 0.48 +0.9
246,653 + ECD_00216 0.50 +1.4
246,654 - ECD_00216 0.50 -0.8
246,654 - ECD_00216 0.50 -0.4
246,692 - ECD_00216 0.51 +0.8
246,692 - ECD_00216 0.51 +0.0
246,826 + ECD_00216 0.57 +0.2
246,827 - ECD_00216 0.57 -0.6
247,037 + ECD_00216 0.66 +0.6
247,257 + ECD_00216 0.75 +0.7
247,334 + ECD_00216 0.78 -0.6
247,337 - ECD_00216 0.78 +0.7
247,361 - ECD_00216 0.79 +0.8
247,406 - ECD_00216 0.81 -2.3
247,534 + ECD_00216 0.86 +0.6
247,538 + ECD_00216 0.86 -0.4
247,538 + ECD_00216 0.86 +0.7
247,539 - ECD_00216 0.86 +0.9
247,539 - ECD_00216 0.86 +1.1
247,663 - -0.1
247,686 - +0.5
247,810 - -0.1
247,951 - +0.0
247,974 - +0.4
248,092 + +0.3
248,121 - +1.5
248,377 + ECD_00217 0.45 -0.1
248,776 - +0.8

Or see this region's nucleotide sequence