Strain Fitness in Escherichia coli BL21 around ECD_01432

Experiment: Bas42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01431 and ECD_01432 are separated by 288 nucleotidesECD_01432 and ECD_01433 are separated by 12 nucleotidesECD_01433 and ECD_01434 overlap by 8 nucleotides ECD_01431: ECD_01431 - aromatic amino acid exporter, at 1,501,968 to 1,502,792 _01431 ECD_01432: ECD_01432 - formate dehydrogenase-N, alpha subunit, nitrate-inducible, at 1,503,081 to 1,506,128 _01432 ECD_01433: ECD_01433 - formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible, at 1,506,141 to 1,507,025 _01433 ECD_01434: ECD_01434 - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible, at 1,507,018 to 1,507,671 _01434 Position (kb) 1503 1504 1505 1506 1507Strain fitness (log2 ratio) -1 0 1at 1503.234 kb on + strandat 1503.417 kb on + strand, within ECD_01432at 1503.452 kb on + strand, within ECD_01432at 1503.560 kb on + strand, within ECD_01432at 1503.993 kb on + strand, within ECD_01432at 1504.698 kb on + strand, within ECD_01432at 1504.955 kb on + strand, within ECD_01432at 1504.955 kb on + strand, within ECD_01432at 1505.158 kb on + strand, within ECD_01432at 1505.369 kb on + strand, within ECD_01432at 1505.415 kb on + strand, within ECD_01432at 1505.763 kb on + strand, within ECD_01432at 1505.876 kb on + strandat 1506.612 kb on + strand, within ECD_01433

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas42
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1,503,234 + +0.1
1,503,417 + ECD_01432 0.11 +1.6
1,503,452 + ECD_01432 0.12 -0.9
1,503,560 + ECD_01432 0.16 -0.1
1,503,993 + ECD_01432 0.30 -0.9
1,504,698 + ECD_01432 0.53 +0.7
1,504,955 + ECD_01432 0.61 -0.1
1,504,955 + ECD_01432 0.61 -0.1
1,505,158 + ECD_01432 0.68 -0.4
1,505,369 + ECD_01432 0.75 -1.2
1,505,415 + ECD_01432 0.77 -0.3
1,505,763 + ECD_01432 0.88 -0.4
1,505,876 + -0.7
1,506,612 + ECD_01433 0.53 +1.0

Or see this region's nucleotide sequence