Strain Fitness in Escherichia coli BL21 around ECD_03043

Experiment: Bas32

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03041 and ECD_03042 overlap by 8 nucleotidesECD_03042 and ECD_03043 are separated by 89 nucleotidesECD_03043 and ECD_03044 are separated by 99 nucleotidesECD_03044 and ECD_03045 are separated by 125 nucleotides ECD_03041: ECD_03041 - phosphoglucosamine mutase, at 3,191,232 to 3,192,569 _03041 ECD_03042: ECD_03042 - 7,8-dihydropteroate synthase, at 3,192,562 to 3,193,410 _03042 ECD_03043: ECD_03043 - protease, ATP-dependent zinc-metallo, at 3,193,500 to 3,195,434 _03043 ECD_03044: ECD_03044 - 23S rRNA U2552 2'-O-ribose methyltransferase, SAM-dependent, at 3,195,534 to 3,196,163 _03044 ECD_03045: ECD_03045 - RNA binding protein, at 3,196,289 to 3,196,582 _03045 Position (kb) 3193 3194 3195 3196Strain fitness (log2 ratio) -2 -1 0 1at 3193.436 kb on - strandat 3193.479 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.502 kb on - strandat 3193.513 kb on - strandat 3196.231 kb on - strandat 3196.231 kb on - strandat 3196.285 kb on + strandat 3196.300 kb on + strandat 3196.303 kb on + strandat 3196.303 kb on + strandat 3196.304 kb on - strandat 3196.304 kb on - strandat 3196.367 kb on - strand, within ECD_03045at 3196.411 kb on - strand, within ECD_03045

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas32
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3,193,436 - -0.2
3,193,479 - +0.5
3,193,502 - -0.0
3,193,502 - +0.2
3,193,502 - -1.8
3,193,502 - -0.0
3,193,502 - -0.9
3,193,513 - +0.7
3,196,231 - -1.8
3,196,231 - -1.2
3,196,285 + +0.2
3,196,300 + -1.8
3,196,303 + -0.2
3,196,303 + -1.7
3,196,304 - +0.7
3,196,304 - +0.6
3,196,367 - ECD_03045 0.27 +0.0
3,196,411 - ECD_03045 0.41 +0.9

Or see this region's nucleotide sequence