Experiment: Bas32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_03041 and ECD_03042 overlap by 8 nucleotides ECD_03042 and ECD_03043 are separated by 89 nucleotides ECD_03043 and ECD_03044 are separated by 99 nucleotides ECD_03044 and ECD_03045 are separated by 125 nucleotides
ECD_03041: ECD_03041 - phosphoglucosamine mutase, at 3,191,232 to 3,192,569
_03041
ECD_03042: ECD_03042 - 7,8-dihydropteroate synthase, at 3,192,562 to 3,193,410
_03042
ECD_03043: ECD_03043 - protease, ATP-dependent zinc-metallo, at 3,193,500 to 3,195,434
_03043
ECD_03044: ECD_03044 - 23S rRNA U2552 2'-O-ribose methyltransferase, SAM-dependent, at 3,195,534 to 3,196,163
_03044
ECD_03045: ECD_03045 - RNA binding protein, at 3,196,289 to 3,196,582
_03045
Position (kb)
3193
3194
3195
3196 Strain fitness (log2 ratio)
-2
-1
0
1 at 3193.436 kb on - strand at 3193.479 kb on - strand at 3193.502 kb on - strand at 3193.502 kb on - strand at 3193.502 kb on - strand at 3193.502 kb on - strand at 3193.502 kb on - strand at 3193.513 kb on - strand at 3196.231 kb on - strand at 3196.231 kb on - strand at 3196.285 kb on + strand at 3196.300 kb on + strand at 3196.303 kb on + strand at 3196.303 kb on + strand at 3196.304 kb on - strand at 3196.304 kb on - strand at 3196.367 kb on - strand, within ECD_03045 at 3196.411 kb on - strand, within ECD_03045
Per-strain Table
Position Strand Gene LocusTag Fraction Bas32 remove 3,193,436 - -0.2 3,193,479 - +0.5 3,193,502 - -0.0 3,193,502 - +0.2 3,193,502 - -1.8 3,193,502 - -0.0 3,193,502 - -0.9 3,193,513 - +0.7 3,196,231 - -1.8 3,196,231 - -1.2 3,196,285 + +0.2 3,196,300 + -1.8 3,196,303 + -0.2 3,196,303 + -1.7 3,196,304 - +0.7 3,196,304 - +0.6 3,196,367 - ECD_03045 0.27 +0.0 3,196,411 - ECD_03045 0.41 +0.9
Or see this region's nucleotide sequence