Experiment: Bas32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00112 and ECD_00113 are separated by 160 nucleotides ECD_00113 and ECD_00114 are separated by 14 nucleotides
ECD_00112: ECD_00112 - pyruvate dehydrogenase complex repressor; autorepressor, at 124,897 to 125,661
_00112
ECD_00113: ECD_00113 - pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate-binding, at 125,822 to 128,485
_00113
ECD_00114: ECD_00114 - pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2, at 128,500 to 130,392
_00114
Position (kb) 125
126
127
128
129 Strain fitness (log2 ratio) -2
-1
0
1
2 at 124.883 kb on + strand at 124.903 kb on + strand at 124.903 kb on + strand at 125.188 kb on + strand, within ECD_00112 at 125.188 kb on + strand, within ECD_00112 at 125.188 kb on + strand, within ECD_00112 at 125.223 kb on + strand, within ECD_00112 at 125.233 kb on + strand, within ECD_00112 at 125.236 kb on + strand, within ECD_00112 at 125.405 kb on + strand, within ECD_00112 at 125.412 kb on + strand, within ECD_00112 at 125.412 kb on + strand, within ECD_00112 at 125.412 kb on + strand, within ECD_00112 at 125.412 kb on + strand, within ECD_00112 at 125.412 kb on + strand, within ECD_00112 at 125.461 kb on + strand, within ECD_00112 at 125.510 kb on + strand, within ECD_00112 at 125.733 kb on + strand at 127.172 kb on + strand, within ECD_00113 at 127.856 kb on + strand, within ECD_00113
Per-strain Table
Position Strand Gene LocusTag Fraction Bas32 remove 124,883 + -0.3 124,903 + -0.7 124,903 + -0.2 125,188 + ECD_00112 0.38 -0.1 125,188 + ECD_00112 0.38 -0.5 125,188 + ECD_00112 0.38 +2.0 125,223 + ECD_00112 0.43 -1.1 125,233 + ECD_00112 0.44 -0.4 125,236 + ECD_00112 0.44 -2.0 125,405 + ECD_00112 0.66 -0.0 125,412 + ECD_00112 0.67 -0.8 125,412 + ECD_00112 0.67 -0.3 125,412 + ECD_00112 0.67 -0.7 125,412 + ECD_00112 0.67 -0.6 125,412 + ECD_00112 0.67 -2.0 125,461 + ECD_00112 0.74 -0.1 125,510 + ECD_00112 0.80 -0.1 125,733 + -0.4 127,172 + ECD_00113 0.51 +1.6 127,856 + ECD_00113 0.76 +1.6
Or see this region's nucleotide sequence