Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1368 and Psyr_1369 are separated by 53 nucleotides Psyr_1369 and Psyr_1370 overlap by 4 nucleotides Psyr_1370 and Psyr_1371 overlap by 13 nucleotides Psyr_1371 and Psyr_1372 overlap by 1 nucleotides
Psyr_1368: Psyr_1368 - Carboxylesterase, at 1,551,681 to 1,552,526
_1368
Psyr_1369: Psyr_1369 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,552,580 to 1,553,053
_1369
Psyr_1370: Psyr_1370 - tRNA pseudouridine synthase D, TruD, at 1,553,050 to 1,554,108
_1370
Psyr_1371: Psyr_1371 - 5'-nucleotidase / exopolyphosphatase / 3'-nucleotidase, at 1,554,096 to 1,554,845
_1371
Psyr_1372: Psyr_1372 - Protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 1,554,845 to 1,555,522
_1372
Position (kb)
1553
1554
1555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1552.053 kb on + strand, within Psyr_1368 at 1552.053 kb on + strand, within Psyr_1368 at 1552.115 kb on + strand, within Psyr_1368 at 1552.255 kb on - strand, within Psyr_1368 at 1552.461 kb on + strand at 1552.461 kb on + strand at 1552.461 kb on + strand at 1552.462 kb on - strand at 1552.462 kb on - strand at 1552.462 kb on - strand at 1552.543 kb on + strand at 1553.084 kb on - strand at 1553.275 kb on + strand, within Psyr_1370 at 1553.530 kb on + strand, within Psyr_1370 at 1553.531 kb on - strand, within Psyr_1370 at 1553.587 kb on + strand, within Psyr_1370 at 1553.656 kb on + strand, within Psyr_1370 at 1553.657 kb on - strand, within Psyr_1370 at 1553.657 kb on - strand, within Psyr_1370 at 1553.878 kb on - strand, within Psyr_1370 at 1553.983 kb on + strand, within Psyr_1370 at 1554.101 kb on + strand at 1554.102 kb on - strand at 1554.102 kb on - strand at 1554.109 kb on + strand at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.394 kb on + strand, within Psyr_1371 at 1554.395 kb on - strand, within Psyr_1371 at 1554.395 kb on - strand, within Psyr_1371 at 1554.395 kb on - strand, within Psyr_1371 at 1554.395 kb on - strand, within Psyr_1371 at 1554.395 kb on - strand, within Psyr_1371 at 1554.508 kb on - strand, within Psyr_1371 at 1554.576 kb on - strand, within Psyr_1371 at 1554.751 kb on + strand, within Psyr_1371 at 1554.751 kb on + strand, within Psyr_1371 at 1554.751 kb on + strand, within Psyr_1371 at 1554.759 kb on + strand, within Psyr_1371 at 1554.760 kb on - strand, within Psyr_1371 at 1554.764 kb on - strand, within Psyr_1371 at 1554.764 kb on - strand, within Psyr_1371 at 1554.867 kb on + strand at 1554.868 kb on - strand at 1554.868 kb on - strand at 1555.073 kb on + strand, within Psyr_1372 at 1555.073 kb on + strand, within Psyr_1372 at 1555.073 kb on + strand, within Psyr_1372 at 1555.073 kb on + strand, within Psyr_1372 at 1555.073 kb on + strand, within Psyr_1372 at 1555.073 kb on + strand, within Psyr_1372 at 1555.074 kb on - strand, within Psyr_1372
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 1,552,053 + Psyr_1368 0.44 +0.2 1,552,053 + Psyr_1368 0.44 +0.5 1,552,115 + Psyr_1368 0.51 +1.7 1,552,255 - Psyr_1368 0.68 +0.6 1,552,461 + +0.3 1,552,461 + +4.2 1,552,461 + +0.0 1,552,462 - +0.7 1,552,462 - +1.7 1,552,462 - +2.7 1,552,543 + +1.2 1,553,084 - +1.7 1,553,275 + Psyr_1370 0.21 -0.2 1,553,530 + Psyr_1370 0.45 +0.3 1,553,531 - Psyr_1370 0.45 +2.4 1,553,587 + Psyr_1370 0.51 +0.9 1,553,656 + Psyr_1370 0.57 +1.4 1,553,657 - Psyr_1370 0.57 -0.5 1,553,657 - Psyr_1370 0.57 -0.9 1,553,878 - Psyr_1370 0.78 -1.2 1,553,983 + Psyr_1370 0.88 -0.0 1,554,101 + +2.7 1,554,102 - +0.2 1,554,102 - +0.6 1,554,109 + -1.8 1,554,394 + Psyr_1371 0.40 -1.7 1,554,394 + Psyr_1371 0.40 -2.0 1,554,394 + Psyr_1371 0.40 -2.0 1,554,394 + Psyr_1371 0.40 +0.3 1,554,394 + Psyr_1371 0.40 -0.9 1,554,394 + Psyr_1371 0.40 -1.7 1,554,394 + Psyr_1371 0.40 -1.1 1,554,394 + Psyr_1371 0.40 -1.0 1,554,394 + Psyr_1371 0.40 -1.0 1,554,395 - Psyr_1371 0.40 -1.6 1,554,395 - Psyr_1371 0.40 -2.3 1,554,395 - Psyr_1371 0.40 -1.7 1,554,395 - Psyr_1371 0.40 -0.5 1,554,395 - Psyr_1371 0.40 -0.2 1,554,508 - Psyr_1371 0.55 -2.0 1,554,576 - Psyr_1371 0.64 -2.8 1,554,751 + Psyr_1371 0.87 -0.7 1,554,751 + Psyr_1371 0.87 -1.1 1,554,751 + Psyr_1371 0.87 -1.4 1,554,759 + Psyr_1371 0.88 -1.0 1,554,760 - Psyr_1371 0.89 -0.6 1,554,764 - Psyr_1371 0.89 -1.9 1,554,764 - Psyr_1371 0.89 -1.5 1,554,867 + -1.8 1,554,868 - -1.1 1,554,868 - -1.3 1,555,073 + Psyr_1372 0.34 -1.4 1,555,073 + Psyr_1372 0.34 +0.6 1,555,073 + Psyr_1372 0.34 -0.8 1,555,073 + Psyr_1372 0.34 -1.3 1,555,073 + Psyr_1372 0.34 -0.5 1,555,073 + Psyr_1372 0.34 -1.8 1,555,074 - Psyr_1372 0.34 +0.3
Or see this region's nucleotide sequence