Experiment: KB with Conessine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0460 and Psyr_0461 are separated by 354 nucleotides Psyr_0461 and Psyr_0462 are separated by 152 nucleotides Psyr_0462 and Psyr_0463 are separated by 22 nucleotides
Psyr_0460: Psyr_0460 - adenosylhomocysteinase, at 498,180 to 499,589
_0460
Psyr_0461: Psyr_0461 - Thioesterase superfamily, at 499,944 to 500,339
_0461
Psyr_0462: Psyr_0462 - Hydroxypyruvate isomerase, at 500,492 to 501,277
_0462
Psyr_0463: Psyr_0463 - Gluconate transporter, at 501,300 to 502,757
_0463
Position (kb)
499
500
501 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 499.746 kb on + strand at 499.746 kb on + strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.747 kb on - strand at 499.775 kb on - strand at 499.816 kb on + strand at 499.844 kb on + strand at 499.847 kb on + strand at 499.848 kb on - strand at 499.867 kb on + strand at 499.915 kb on - strand at 500.049 kb on + strand, within Psyr_0461 at 500.050 kb on - strand, within Psyr_0461 at 500.050 kb on - strand, within Psyr_0461 at 500.070 kb on - strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.091 kb on + strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.092 kb on - strand, within Psyr_0461 at 500.200 kb on + strand, within Psyr_0461 at 500.201 kb on - strand, within Psyr_0461 at 500.330 kb on - strand at 500.330 kb on - strand at 500.330 kb on - strand at 500.330 kb on - strand at 500.376 kb on + strand at 500.417 kb on + strand at 500.418 kb on - strand at 500.418 kb on - strand at 500.418 kb on - strand at 500.480 kb on + strand at 500.580 kb on + strand, within Psyr_0462 at 500.617 kb on - strand, within Psyr_0462 at 500.914 kb on - strand, within Psyr_0462 at 500.914 kb on - strand, within Psyr_0462 at 500.914 kb on - strand, within Psyr_0462 at 501.001 kb on - strand, within Psyr_0462 at 501.159 kb on + strand, within Psyr_0462 at 501.159 kb on + strand, within Psyr_0462 at 501.159 kb on + strand, within Psyr_0462 at 501.160 kb on - strand, within Psyr_0462 at 501.160 kb on - strand, within Psyr_0462 at 501.160 kb on - strand, within Psyr_0462 at 501.160 kb on - strand, within Psyr_0462 at 501.160 kb on - strand, within Psyr_0462 at 501.177 kb on + strand, within Psyr_0462 at 501.177 kb on + strand, within Psyr_0462 at 501.178 kb on - strand, within Psyr_0462 at 501.280 kb on + strand at 501.280 kb on + strand at 501.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Conessine 10 mM remove 499,746 + +0.7 499,746 + -1.7 499,747 - +1.5 499,747 - -0.2 499,747 - -0.4 499,747 - +0.4 499,775 - +0.6 499,816 + +2.5 499,844 + +1.1 499,847 + +1.8 499,848 - +0.6 499,867 + +1.5 499,915 - -0.7 500,049 + Psyr_0461 0.27 -0.2 500,050 - Psyr_0461 0.27 -0.3 500,050 - Psyr_0461 0.27 +0.9 500,070 - Psyr_0461 0.32 +0.1 500,091 + Psyr_0461 0.37 -0.4 500,091 + Psyr_0461 0.37 +0.1 500,091 + Psyr_0461 0.37 +1.0 500,091 + Psyr_0461 0.37 -0.6 500,091 + Psyr_0461 0.37 -2.0 500,091 + Psyr_0461 0.37 -0.6 500,091 + Psyr_0461 0.37 +0.8 500,091 + Psyr_0461 0.37 +0.8 500,091 + Psyr_0461 0.37 +3.1 500,091 + Psyr_0461 0.37 -4.0 500,091 + Psyr_0461 0.37 +0.1 500,091 + Psyr_0461 0.37 +0.1 500,091 + Psyr_0461 0.37 -0.5 500,091 + Psyr_0461 0.37 -0.6 500,092 - Psyr_0461 0.37 -0.5 500,092 - Psyr_0461 0.37 -0.6 500,092 - Psyr_0461 0.37 +0.7 500,092 - Psyr_0461 0.37 +0.1 500,092 - Psyr_0461 0.37 -0.3 500,092 - Psyr_0461 0.37 -0.9 500,092 - Psyr_0461 0.37 +0.6 500,092 - Psyr_0461 0.37 -0.7 500,092 - Psyr_0461 0.37 -0.9 500,092 - Psyr_0461 0.37 +0.1 500,092 - Psyr_0461 0.37 +0.2 500,092 - Psyr_0461 0.37 -0.6 500,092 - Psyr_0461 0.37 +2.0 500,092 - Psyr_0461 0.37 +1.0 500,092 - Psyr_0461 0.37 -2.3 500,200 + Psyr_0461 0.65 +1.9 500,201 - Psyr_0461 0.65 +1.1 500,330 - -0.2 500,330 - -1.1 500,330 - -2.2 500,330 - -0.4 500,376 + +0.5 500,417 + -0.6 500,418 - +1.3 500,418 - -0.2 500,418 - -0.6 500,480 + +0.8 500,580 + Psyr_0462 0.11 +2.7 500,617 - Psyr_0462 0.16 +1.5 500,914 - Psyr_0462 0.54 +2.5 500,914 - Psyr_0462 0.54 -0.3 500,914 - Psyr_0462 0.54 -0.1 501,001 - Psyr_0462 0.65 -0.8 501,159 + Psyr_0462 0.85 -0.6 501,159 + Psyr_0462 0.85 +1.9 501,159 + Psyr_0462 0.85 -0.4 501,160 - Psyr_0462 0.85 +0.9 501,160 - Psyr_0462 0.85 +0.5 501,160 - Psyr_0462 0.85 -0.3 501,160 - Psyr_0462 0.85 -0.2 501,160 - Psyr_0462 0.85 -0.2 501,177 + Psyr_0462 0.87 -0.7 501,177 + Psyr_0462 0.87 +0.1 501,178 - Psyr_0462 0.87 -0.6 501,280 + -1.1 501,280 + +0.4 501,280 + +1.1
Or see this region's nucleotide sequence